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• W2103552974 endingPage "9567" @default.
• W2103552974 startingPage "9557" @default.
• W2103552974 abstract "It has been suggested that the Schizosaccharomyces pombe Rad50 (Rad50-Rad32-Nbs1) complex is required for the resection of the C-rich strand at telomere ends in taz1-d cells. However, the nuclease-deficient Rad32-D25A mutant can still resect the C-rich strand, suggesting the existence of a nuclease that resects the C-rich strand. Here, we demonstrate that a taz1-d dna2-2C double mutant lost the G-rich overhang at a semipermissive temperature. The amount of G-rich overhang in S phase in the dna2-C2 mutant was lower than that in wild-type cells at the semipermissive temperature. Dna2 bound to telomere DNA in a chromatin immunoprecipitation assay. Moreover, telomere length decreased with each generation after shift of the dna2-2C mutant to the semipermissive temperature. These results suggest that Dna2 is involved in the generation of G-rich overhangs in both wild-type cells and taz1-d cells. The dna2-C2 mutant was not gamma ray sensitive at the semipermissive temperature, suggesting that the ability to process double-strand break (DSB) ends was not affected in the dna2-C2 mutant. Our results reveal that DSB ends and telomere ends are processed by different mechanisms." @default.
• W2103552974 created "2016-06-24" @default.
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• W2103552974 creator A5056863207 @default.
• W2103552974 creator A5061544428 @default.
• W2103552974 date "2004-11-01" @default.
• W2103552974 modified "2023-09-23" @default.
• W2103552974 title "Fission Yeast Dna2 Is Required for Generation of the Telomeric Single-Strand Overhang" @default.
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• W2103552974 doi "https://doi.org/10.1128/mcb.24.21.9557-9567.2004" @default.
• W2103552974 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/522233" @default.
• W2103552974 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15485922" @default.
• W2103552974 hasPublicationYear "2004" @default.
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