FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103560063> ?p ?o ?g. }

• W2103560063 endingPage "1164" @default.
• W2103560063 startingPage "1157" @default.
• W2103560063 abstract "We have recently developed novel high-affinity blockers for the dopamine transporter (DAT) by carrying out structure-activity studies of GBR 12909 molecule piperidine analogs. To investigate the molecular basis of binding of these compounds in comparison to known sites of action of GBR 12909, cocaine, and benztropine analogs, we developed a piperidine-based photoaffinity label [(125)I]4-[2-(diphenylmethoxy)ethyl]-1-[(4-azido- 3-iodophenyl)methyl]-piperidine [(125)I]AD-96-129), and used proteolysis and epitope-specific immunoprecipitation to identify the protein domains that interact with the ligand. [(125)I]AD-96-129 became incorporated into two different regions of the DAT primary sequence, an N-terminal site containing transmembrane domains (TMs) 1 to 2, and a second site containing TMs 4 to 6. Both of these regions have been identified previously as sites involved in the binding of other DAT photoaffinity labels. However, in contrast to the previously characterized ligands that showed nearly complete specificity in their binding site incorporation, [(125)I]AD-96-129 became incorporated into both sites at comparable levels. These results suggest that the two domains may be in close three-dimensional proximity and contribute to binding of multiple uptake blockers. We also found that DATs labeled with [(125)I]AD-96-129 or other photoaffinity labels displayed distinctive sensitivities to proteolysis of a site in the second extracellular loop, with protease resistance related to the extent of ligand incorporation in the TM4 to 6 region. These differences in protease sensitivity may indicate the relative proximity of the ligands to the protease site or reflect antagonist-induced conformational changes in the loop related to transport inhibition." @default.
• W2103560063 created "2016-06-24" @default.
• W2103560063 creator A5018939644 @default.
• W2103560063 creator A5020951431 @default.
• W2103560063 creator A5045041243 @default.
• W2103560063 creator A5089602272 @default.
• W2103560063 creator A5091767306 @default.
• W2103560063 date "2001-05-01" @default.
• W2103560063 modified "2023-09-29" @default.
• W2103560063 title "Dual Incorporation of Photoaffinity Ligands on Dopamine Transporters Implicates Proximity of Labeled Domains" @default.
• W2103560063 cites W1506737211 @default.
• W2103560063 cites W1514724839 @default.
• W2103560063 cites W1591356314 @default.
• W2103560063 cites W1796402873 @default.
• W2103560063 cites W1877347825 @default.
• W2103560063 cites W1966183574 @default.
• W2103560063 cites W1979607966 @default.
• W2103560063 cites W1985442468 @default.
• W2103560063 cites W2006203019 @default.
• W2103560063 cites W2007604257 @default.
• W2103560063 cites W2015379298 @default.
• W2103560063 cites W2020727025 @default.
• W2103560063 cites W2021382411 @default.
• W2103560063 cites W2023218084 @default.
• W2103560063 cites W2029516351 @default.
• W2103560063 cites W2029746799 @default.
• W2103560063 cites W2030930964 @default.
• W2103560063 cites W2049055505 @default.
• W2103560063 cites W2055939533 @default.
• W2103560063 cites W2062133534 @default.
• W2103560063 cites W2063752616 @default.
• W2103560063 cites W2070147672 @default.
• W2103560063 cites W2072505484 @default.
• W2103560063 cites W2075347406 @default.
• W2103560063 cites W2078744556 @default.
• W2103560063 cites W2082450996 @default.
• W2103560063 cites W2094023488 @default.
• W2103560063 cites W2100268743 @default.
• W2103560063 cites W2168959672 @default.
• W2103560063 cites W294896375 @default.
• W2103560063 cites W2950828283 @default.
• W2103560063 cites W37645710 @default.
• W2103560063 cites W4229528378 @default.
• W2103560063 cites W74063644 @default.
• W2103560063 doi "https://doi.org/10.1124/mol.59.5.1157" @default.
• W2103560063 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11306699" @default.
• W2103560063 hasPublicationYear "2001" @default.
• W2103560063 type Work @default.
• W2103560063 sameAs 2103560063 @default.
• W2103560063 citedByCount "30" @default.
• W2103560063 countsByYear W21035600632014 @default.
• W2103560063 crossrefType "journal-article" @default.
• W2103560063 hasAuthorship W2103560063A5018939644 @default.
• W2103560063 hasAuthorship W2103560063A5020951431 @default.
• W2103560063 hasAuthorship W2103560063A5045041243 @default.
• W2103560063 hasAuthorship W2103560063A5089602272 @default.
• W2103560063 hasAuthorship W2103560063A5091767306 @default.
• W2103560063 hasConcept C104317684 @default.
• W2103560063 hasConcept C107824862 @default.
• W2103560063 hasConcept C116569031 @default.
• W2103560063 hasConcept C118892022 @default.
• W2103560063 hasConcept C149011108 @default.
• W2103560063 hasConcept C170493617 @default.
• W2103560063 hasConcept C181199279 @default.
• W2103560063 hasConcept C185592680 @default.
• W2103560063 hasConcept C2776714187 @default.
• W2103560063 hasConcept C2776755682 @default.
• W2103560063 hasConcept C2778581200 @default.
• W2103560063 hasConcept C2781307694 @default.
• W2103560063 hasConcept C2909816965 @default.
• W2103560063 hasConcept C55493867 @default.
• W2103560063 hasConcept C71240020 @default.
• W2103560063 hasConceptScore W2103560063C104317684 @default.
• W2103560063 hasConceptScore W2103560063C107824862 @default.
• W2103560063 hasConceptScore W2103560063C116569031 @default.
• W2103560063 hasConceptScore W2103560063C118892022 @default.
• W2103560063 hasConceptScore W2103560063C149011108 @default.
• W2103560063 hasConceptScore W2103560063C170493617 @default.
• W2103560063 hasConceptScore W2103560063C181199279 @default.
• W2103560063 hasConceptScore W2103560063C185592680 @default.
• W2103560063 hasConceptScore W2103560063C2776714187 @default.
• W2103560063 hasConceptScore W2103560063C2776755682 @default.
• W2103560063 hasConceptScore W2103560063C2778581200 @default.
• W2103560063 hasConceptScore W2103560063C2781307694 @default.
• W2103560063 hasConceptScore W2103560063C2909816965 @default.
• W2103560063 hasConceptScore W2103560063C55493867 @default.
• W2103560063 hasConceptScore W2103560063C71240020 @default.
• W2103560063 hasIssue "5" @default.
• W2103560063 hasLocation W21035600631 @default.
• W2103560063 hasLocation W21035600632 @default.
• W2103560063 hasOpenAccess W2103560063 @default.
• W2103560063 hasPrimaryLocation W21035600631 @default.
• W2103560063 hasRelatedWork W1984704777 @default.
• W2103560063 hasRelatedWork W2002204277 @default.
• W2103560063 hasRelatedWork W2019647316 @default.
• W2103560063 hasRelatedWork W2023218084 @default.
• W2103560063 hasRelatedWork W2044609389 @default.
• W2103560063 hasRelatedWork W2071436084 @default.

• next