FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103586220> ?p ?o ?g. }

• W2103586220 endingPage "129" @default.
• W2103586220 startingPage "129" @default.
• W2103586220 abstract "The multifunctional polypeptide cyclosporin synthetase (CySyn) remains one of the most complex nonribosomal peptide synthetase described. In this study we used a highly specific photoaffinity labeling procedure with the natural cofactor S-adenosyl-L-methionine (AdoMet), 14C-isotopically labeled at the Sdelta methyl group to probe the concerted AdoMet-binding interaction of the N-methyltransferase (N-MTase) centers of CySyn. The binding stoichiometry for the enzyme-AdoMet complex was determined to be 1:7, which is in agreement with inferences made from analysis of the complementary DNA sequence of the simA gene encoding the CySyn polypeptide. The photolabeling of the AdoMet-binding sites displayed homotropic negative cooperativity, characterized by a curvilinear Scatchard plot with upward concavity. Although, the process of N-methyl transfer is not a critical event for peptide elongation, the destabilizing homotropic interactions between N-MTase centers that were observed may represent a mechanism whereby the enzyme preserves the proficiency of the substrate-channeling process of cyclosporin peptide assembly over a broad range of cofactor concentrations. Furthermore, we demonstrated the utility of the photolabeling procedure for tracking the enzyme during purification." @default.
• W2103586220 created "2016-06-24" @default.
• W2103586220 creator A5020748618 @default.
• W2103586220 creator A5074972614 @default.
• W2103586220 date "2003-01-01" @default.
• W2103586220 modified "2023-09-30" @default.
• W2103586220 title "Photoaffinity Labeling of the N-methyltransferase Domains of Cyclosporin Synthetase¶" @default.
• W2103586220 cites W1547477601 @default.
• W2103586220 cites W1581129475 @default.
• W2103586220 cites W1584186345 @default.
• W2103586220 cites W1596255677 @default.
• W2103586220 cites W1608216620 @default.
• W2103586220 cites W1964516896 @default.
• W2103586220 cites W1975538376 @default.
• W2103586220 cites W1979085852 @default.
• W2103586220 cites W1983649348 @default.
• W2103586220 cites W1990783608 @default.
• W2103586220 cites W1996256335 @default.
• W2103586220 cites W2001396254 @default.
• W2103586220 cites W2005392755 @default.
• W2103586220 cites W2006986579 @default.
• W2103586220 cites W2009652285 @default.
• W2103586220 cites W2013612636 @default.
• W2103586220 cites W2016046073 @default.
• W2103586220 cites W2038606788 @default.
• W2103586220 cites W2046213778 @default.
• W2103586220 cites W2046283710 @default.
• W2103586220 cites W2047261739 @default.
• W2103586220 cites W2057766174 @default.
• W2103586220 cites W2057850803 @default.
• W2103586220 cites W2069950998 @default.
• W2103586220 cites W2074581799 @default.
• W2103586220 cites W2074631079 @default.
• W2103586220 cites W2075561057 @default.
• W2103586220 cites W2089475202 @default.
• W2103586220 cites W2095862645 @default.
• W2103586220 cites W2100837269 @default.
• W2103586220 cites W2134353698 @default.
• W2103586220 cites W4293247451 @default.
• W2103586220 doi "https://doi.org/10.1562/0031-8655(2003)077<0129:plotnm>2.0.co;2" @default.
• W2103586220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12785050" @default.
• W2103586220 hasPublicationYear "2003" @default.
• W2103586220 type Work @default.
• W2103586220 sameAs 2103586220 @default.
• W2103586220 citedByCount "8" @default.
• W2103586220 countsByYear W21035862202012 @default.
• W2103586220 countsByYear W21035862202014 @default.
• W2103586220 countsByYear W21035862202015 @default.
• W2103586220 crossrefType "journal-article" @default.
• W2103586220 hasAuthorship W2103586220A5020748618 @default.
• W2103586220 hasAuthorship W2103586220A5074972614 @default.
• W2103586220 hasConcept C107824862 @default.
• W2103586220 hasConcept C166014724 @default.
• W2103586220 hasConcept C181199279 @default.
• W2103586220 hasConcept C185592680 @default.
• W2103586220 hasConcept C187250156 @default.
• W2103586220 hasConcept C197957613 @default.
• W2103586220 hasConcept C2777379556 @default.
• W2103586220 hasConcept C2778581200 @default.
• W2103586220 hasConcept C2779281246 @default.
• W2103586220 hasConcept C33288867 @default.
• W2103586220 hasConcept C552990157 @default.
• W2103586220 hasConcept C553450214 @default.
• W2103586220 hasConcept C55493867 @default.
• W2103586220 hasConcept C71240020 @default.
• W2103586220 hasConcept C91965660 @default.
• W2103586220 hasConceptScore W2103586220C107824862 @default.
• W2103586220 hasConceptScore W2103586220C166014724 @default.
• W2103586220 hasConceptScore W2103586220C181199279 @default.
• W2103586220 hasConceptScore W2103586220C185592680 @default.
• W2103586220 hasConceptScore W2103586220C187250156 @default.
• W2103586220 hasConceptScore W2103586220C197957613 @default.
• W2103586220 hasConceptScore W2103586220C2777379556 @default.
• W2103586220 hasConceptScore W2103586220C2778581200 @default.
• W2103586220 hasConceptScore W2103586220C2779281246 @default.
• W2103586220 hasConceptScore W2103586220C33288867 @default.
• W2103586220 hasConceptScore W2103586220C552990157 @default.
• W2103586220 hasConceptScore W2103586220C553450214 @default.
• W2103586220 hasConceptScore W2103586220C55493867 @default.
• W2103586220 hasConceptScore W2103586220C71240020 @default.
• W2103586220 hasConceptScore W2103586220C91965660 @default.
• W2103586220 hasIssue "2" @default.
• W2103586220 hasLocation W21035862201 @default.
• W2103586220 hasLocation W21035862202 @default.
• W2103586220 hasOpenAccess W2103586220 @default.
• W2103586220 hasPrimaryLocation W21035862201 @default.
• W2103586220 hasRelatedWork W1004292963 @default.
• W2103586220 hasRelatedWork W1520771983 @default.
• W2103586220 hasRelatedWork W1534300168 @default.
• W2103586220 hasRelatedWork W1977392864 @default.
• W2103586220 hasRelatedWork W1986169335 @default.
• W2103586220 hasRelatedWork W2023858122 @default.
• W2103586220 hasRelatedWork W2893262868 @default.
• W2103586220 hasRelatedWork W3131469594 @default.
• W2103586220 hasRelatedWork W4245431867 @default.
• W2103586220 hasRelatedWork W2552428624 @default.
• W2103586220 hasVolume "77" @default.
• W2103586220 isParatext "false" @default.

• next