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• W2103660187 abstract "Chromatin immunoprecipitation (ChIP) is widely used in many fields to analyze the distribution of specific proteins, or their modified isoforms, across defined DNA domains. ChIP procedures fall into two main categories, namely, those that use native chromatin prepared by nuclease digestion (designated NChIP), and those that use chromatin in which DNA and proteins are crosslinked, either chemically or with UV light (designated XChIP). Each procedure has its own advantages and drawbacks. Here, we outline the methods currently in use in our laboratory to isolate and immunoprecipitate native chromatin from cultured cells, and to isolate and analyze immunoprecipitated protein and DNA." @default.
• W2103660187 created "2016-06-24" @default.
• W2103660187 creator A5077753928 @default.
• W2103660187 creator A5085047665 @default.
• W2103660187 date "2003-09-01" @default.
• W2103660187 modified "2023-10-17" @default.
• W2103660187 title "Immunoprecipitation of native chromatin: NChIP" @default.
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• W2103660187 doi "https://doi.org/10.1016/s1046-2023(03)00090-2" @default.
• W2103660187 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12893176" @default.
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