FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103672917> ?p ?o ?g. }

• W2103672917 endingPage "636" @default.
• W2103672917 startingPage "628" @default.
• W2103672917 abstract "Northern blotting, in situ hybridization, and oligodeoxyribonucleotide excess solution hybridization were used to quantitate metallothionein-I (MT-I) and MT-II mRNAs in mouse testes. Testes from sexually mature adults contained high levels of both MT mRNAs (approximately 10-fold higher than those in control adult liver). Testicular MT mRNA levels were age dependent, being low the first 2 weeks after birth and increasing slowly thereafter to maximal levels in the adult (by 9 weeks after birth). In the adult testis, in situ hybridization indicated that only cells within the adluminal compartment (germ cells) of the seminiferous tubules contain high levels of MT mRNA. The appearance of cells containing elevated levels of MT mRNA during development was delayed from the onset of spermatogenesis. In situ hybridization suggested that MT mRNA accumulates after the initial differentiation of primary spermatocytes and is maintained in spermatids. Pachytene spermatocytes (PSC) and round spermatids (RTD) isolated from adult testes contained both MT-I and MT-II mRNAs in levels equivalent to those found in zinc-treated hepatocytes, whereas very low levels of MT mRNA were detected in isolated Sertoli cells (ST). In situ hybridization suggested that MT mRNA was present at only basal levels in interstitial, spermatogonial, and mature sperm cells at all developmental stages examined. Northern blot and in situ hybridization to sulfated glycoprotein-2 (SGP-2) mRNA, a ST-specific transcript, showed that SGP-2 mRNA is high in the testis of 1-week-old mice and decreases gradually to a lower level in the adult. In situ detection of this mRNA was consistent with the location of ST in the testis. SGP-2 mRNA was abundant in ST and rare in PSC and RTD preparations. Analysis of pulse-labeled proteins from isolated PSC and RTD indicated that these cells actively synthesize MT-I and MT-II. The high levels of MT mRNA in adult testes were not increased substantially after systemic injection of cadmium, zinc, or bacterial lipopolysaccharide. In marked contrast, these treatments led to dramatically increased levels of hepatic and ovarian MT mRNA. This study establishes that the MT genes are actively expressed in a developmentally regulated fashion in the male germ cells of the mouse. This suggests a role for MT in the process of spermatogenesis." @default.
• W2103672917 created "2016-06-24" @default.
• W2103672917 creator A5025588629 @default.
• W2103672917 creator A5054997692 @default.
• W2103672917 creator A5063900337 @default.
• W2103672917 date "1991-05-01" @default.
• W2103672917 modified "2023-10-12" @default.
• W2103672917 title "High Levels of Metallothionein Messenger RNAs in Male Germ Cells of the Adult Mouse" @default.
• W2103672917 cites W122237346 @default.
• W2103672917 cites W1248664554 @default.
• W2103672917 cites W1521702495 @default.
• W2103672917 cites W152871635 @default.
• W2103672917 cites W1538199768 @default.
• W2103672917 cites W1586975947 @default.
• W2103672917 cites W1604713610 @default.
• W2103672917 cites W1806544363 @default.
• W2103672917 cites W1822999471 @default.
• W2103672917 cites W1895321601 @default.
• W2103672917 cites W1926513843 @default.
• W2103672917 cites W1989224162 @default.
• W2103672917 cites W19893031 @default.
• W2103672917 cites W2005409372 @default.
• W2103672917 cites W2007972409 @default.
• W2103672917 cites W2021008729 @default.
• W2103672917 cites W2023256437 @default.
• W2103672917 cites W2024838528 @default.
• W2103672917 cites W2032458072 @default.
• W2103672917 cites W2054491767 @default.
• W2103672917 cites W2056917827 @default.
• W2103672917 cites W2058953608 @default.
• W2103672917 cites W2071709406 @default.
• W2103672917 cites W2078119356 @default.
• W2103672917 cites W2082025200 @default.
• W2103672917 cites W2092654721 @default.
• W2103672917 cites W2105975166 @default.
• W2103672917 cites W2126393573 @default.
• W2103672917 cites W2130939892 @default.
• W2103672917 cites W2155601610 @default.
• W2103672917 cites W2157600274 @default.
• W2103672917 cites W2159520029 @default.
• W2103672917 cites W2162288856 @default.
• W2103672917 cites W2200538274 @default.
• W2103672917 cites W2273089598 @default.
• W2103672917 cites W2282116446 @default.
• W2103672917 cites W2321378101 @default.
• W2103672917 cites W2403221567 @default.
• W2103672917 cites W2417747624 @default.
• W2103672917 cites W253607046 @default.
• W2103672917 cites W561438953 @default.
• W2103672917 doi "https://doi.org/10.1210/mend-5-5-628" @default.
• W2103672917 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2072922" @default.
• W2103672917 hasPublicationYear "1991" @default.
• W2103672917 type Work @default.
• W2103672917 sameAs 2103672917 @default.
• W2103672917 citedByCount "76" @default.
• W2103672917 countsByYear W21036729172012 @default.
• W2103672917 countsByYear W21036729172017 @default.
• W2103672917 countsByYear W21036729172018 @default.
• W2103672917 countsByYear W21036729172019 @default.
• W2103672917 crossrefType "journal-article" @default.
• W2103672917 hasAuthorship W2103672917A5025588629 @default.
• W2103672917 hasAuthorship W2103672917A5054997692 @default.
• W2103672917 hasAuthorship W2103672917A5063900337 @default.
• W2103672917 hasBestOaLocation W21036729171 @default.
• W2103672917 hasConcept C104317684 @default.
• W2103672917 hasConcept C105580179 @default.
• W2103672917 hasConcept C123765429 @default.
• W2103672917 hasConcept C126322002 @default.
• W2103672917 hasConcept C134018914 @default.
• W2103672917 hasConcept C153911025 @default.
• W2103672917 hasConcept C16685009 @default.
• W2103672917 hasConcept C177707886 @default.
• W2103672917 hasConcept C20719415 @default.
• W2103672917 hasConcept C2779066085 @default.
• W2103672917 hasConcept C2781068068 @default.
• W2103672917 hasConcept C54355233 @default.
• W2103672917 hasConcept C71924100 @default.
• W2103672917 hasConcept C81439078 @default.
• W2103672917 hasConcept C86803240 @default.
• W2103672917 hasConceptScore W2103672917C104317684 @default.
• W2103672917 hasConceptScore W2103672917C105580179 @default.
• W2103672917 hasConceptScore W2103672917C123765429 @default.
• W2103672917 hasConceptScore W2103672917C126322002 @default.
• W2103672917 hasConceptScore W2103672917C134018914 @default.
• W2103672917 hasConceptScore W2103672917C153911025 @default.
• W2103672917 hasConceptScore W2103672917C16685009 @default.
• W2103672917 hasConceptScore W2103672917C177707886 @default.
• W2103672917 hasConceptScore W2103672917C20719415 @default.
• W2103672917 hasConceptScore W2103672917C2779066085 @default.
• W2103672917 hasConceptScore W2103672917C2781068068 @default.
• W2103672917 hasConceptScore W2103672917C54355233 @default.
• W2103672917 hasConceptScore W2103672917C71924100 @default.
• W2103672917 hasConceptScore W2103672917C81439078 @default.
• W2103672917 hasConceptScore W2103672917C86803240 @default.
• W2103672917 hasIssue "5" @default.
• W2103672917 hasLocation W21036729171 @default.
• W2103672917 hasLocation W21036729172 @default.
• W2103672917 hasOpenAccess W2103672917 @default.

• next