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• W2103767972 abstract "The finding of new isolates of non-Saccharomyces yeasts, showing beneficial enzymes (such as β-glucosidase and β-xylosidase), can contribute to the production of quality wines. In a selection and characterization program, we have studied 114 isolates of non-Saccharomyces yeasts. Four isolates were selected because of their both high β-glucosidase and β-xylosidase activities. The ribosomal D1/D2 regions were sequenced to identify them as Pichia membranifaciens Pm7, Hanseniaspora vineae Hv3, H. uvarum Hu8, and Wickerhamomyces anomalus Wa1. The induction process was optimized to be carried on YNB-medium supplemented with 4% xylan, inoculated with 106 cfu/mL and incubated 48 h at 28 °C without agitation. Most of the strains had a pH optimum of 5.0 to 6.0 for both the β-glucosidase and β-xylosidase activities. The effect of sugars was different for each isolate and activity. Each isolate showed a characteristic set of inhibition, enhancement or null effect for β-glucosidase and β-xylosidase. The volatile compounds liberated from wine incubated with each of the 4 yeasts were also studied, showing an overall terpene increase (1.1 to 1.3-folds) when wines were treated with non-Saccharomyces isolates. In detail, terpineol, 4-vinyl-phenol and 2-methoxy-4-vinylphenol increased after the addition of Hanseniaspora isolates. Wines treated with Hanseniaspora, Wickerhamomyces, or Pichia produced more 2-phenyl ethanol than those inoculated with other yeasts." @default.
• W2103767972 created "2016-06-24" @default.
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• W2103767972 date "2015-06-30" @default.
• W2103767972 modified "2023-10-03" @default.
• W2103767972 title "Screening of β-Glucosidase and β-Xylosidase Activities in Four Non-<i>Saccharomyces</i>Yeast Isolates" @default.
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• W2103767972 doi "https://doi.org/10.1111/1750-3841.12954" @default.
• W2103767972 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26126488" @default.
• W2103767972 hasPublicationYear "2015" @default.
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