FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2103809350> ?p ?o ?g. }

• W2103809350 endingPage "206" @default.
• W2103809350 startingPage "196" @default.
• W2103809350 abstract "A primary advantage of lentiviral vectors is their ability to pass through the nuclear envelope into the cell nucleus thereby allowing transduction of nondividing cells. Using HIV-based lentiviral vectors, we delivered an anti-CCR5 ribozyme (CCR5RZ), a nucleolar localizing TAR RNA decoy, or Pol III-expressed siRNA genes into cultured and primary cells. The CCR5RZ is driven by the adenoviral VA1 Pol III promoter, while the human U6 snRNA Pol III-transcribed TAR decoy is embedded in a U16 snoRNA (designated U16TAR), and the siRNAs were expressed from the human U6 Pol III promoter. The transduction efficiencies of these vectors ranged from 96-98% in 293 cells to 15-20% in primary PBMCs. A combination of the CCR5RZ and U16TAR decoy in a single vector backbone gave enhanced protection against HIV-1 challenge in a selective survival assay in both primary T cells and CD34(+)-derived monocytes. The lentiviral vector backbone-expressed siRNAs also showed potent inhibition of p24 expression in PBMCs challenged with HIV-1. Overall our results demonstrate that the lentiviral-based vectors can efficiently deliver single constructs as well as combinations of Pol III therapeutic expression units into primary hematopoietic cells for anti-HIV gene therapy and hold promise for stem or T-cell-based gene therapy for HIV-1 infection." @default.
• W2103809350 created "2016-06-24" @default.
• W2103809350 creator A5001187257 @default.
• W2103809350 creator A5021035408 @default.
• W2103809350 creator A5028028396 @default.
• W2103809350 creator A5046038634 @default.
• W2103809350 creator A5054134377 @default.
• W2103809350 creator A5054379102 @default.
• W2103809350 creator A5058854705 @default.
• W2103809350 creator A5061721634 @default.
• W2103809350 creator A5074513417 @default.
• W2103809350 creator A5088904031 @default.
• W2103809350 date "2003-08-01" @default.
• W2103809350 modified "2023-10-16" @default.
• W2103809350 title "Inhibition of HIV-1 infection by lentiviral vectors expressing pol III-promoted anti-HIV RNAs" @default.
• W2103809350 cites W1482247814 @default.
• W2103809350 cites W1488192195 @default.
• W2103809350 cites W1533075742 @default.
• W2103809350 cites W1546402314 @default.
• W2103809350 cites W1587940136 @default.
• W2103809350 cites W1896714280 @default.
• W2103809350 cites W1957422323 @default.
• W2103809350 cites W1964018172 @default.
• W2103809350 cites W1965372133 @default.
• W2103809350 cites W1969034482 @default.
• W2103809350 cites W1969213969 @default.
• W2103809350 cites W1981440980 @default.
• W2103809350 cites W1981449360 @default.
• W2103809350 cites W1981789605 @default.
• W2103809350 cites W1982448824 @default.
• W2103809350 cites W1982765182 @default.
• W2103809350 cites W1986574361 @default.
• W2103809350 cites W1990679519 @default.
• W2103809350 cites W1995953390 @default.
• W2103809350 cites W1996183144 @default.
• W2103809350 cites W1998439550 @default.
• W2103809350 cites W2006765654 @default.
• W2103809350 cites W2007021820 @default.
• W2103809350 cites W2007903872 @default.
• W2103809350 cites W2009412962 @default.
• W2103809350 cites W2009761723 @default.
• W2103809350 cites W2010289001 @default.
• W2103809350 cites W2011767474 @default.
• W2103809350 cites W2020422048 @default.
• W2103809350 cites W2021779296 @default.
• W2103809350 cites W2026102468 @default.
• W2103809350 cites W2037702309 @default.
• W2103809350 cites W2046126827 @default.
• W2103809350 cites W2049856108 @default.
• W2103809350 cites W2056664929 @default.
• W2103809350 cites W2058624126 @default.
• W2103809350 cites W2069071112 @default.
• W2103809350 cites W2071601231 @default.
• W2103809350 cites W2073043894 @default.
• W2103809350 cites W2084851425 @default.
• W2103809350 cites W2088592843 @default.
• W2103809350 cites W2090630729 @default.
• W2103809350 cites W2092575199 @default.
• W2103809350 cites W2105007068 @default.
• W2103809350 cites W2112445703 @default.
• W2103809350 cites W2117025121 @default.
• W2103809350 cites W2117681036 @default.
• W2103809350 cites W2118944608 @default.
• W2103809350 cites W2124026319 @default.
• W2103809350 cites W2126357434 @default.
• W2103809350 cites W2135135539 @default.
• W2103809350 cites W2138230142 @default.
• W2103809350 cites W2146900615 @default.
• W2103809350 cites W2147896158 @default.
• W2103809350 cites W2150505696 @default.
• W2103809350 cites W2161076191 @default.
• W2103809350 cites W2163863526 @default.
• W2103809350 cites W2164653061 @default.
• W2103809350 cites W2171790482 @default.
• W2103809350 cites W2177457327 @default.
• W2103809350 cites W2184551573 @default.
• W2103809350 cites W2318289184 @default.
• W2103809350 cites W3148829076 @default.
• W2103809350 cites W4211039187 @default.
• W2103809350 cites W4254038279 @default.
• W2103809350 cites W4298029310 @default.
• W2103809350 doi "https://doi.org/10.1016/s1525-0016(03)00165-5" @default.
• W2103809350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12907142" @default.
• W2103809350 hasPublicationYear "2003" @default.
• W2103809350 type Work @default.
• W2103809350 sameAs 2103809350 @default.
• W2103809350 citedByCount "150" @default.
• W2103809350 countsByYear W21038093502012 @default.
• W2103809350 countsByYear W21038093502013 @default.
• W2103809350 countsByYear W21038093502014 @default.
• W2103809350 countsByYear W21038093502015 @default.
• W2103809350 countsByYear W21038093502016 @default.
• W2103809350 countsByYear W21038093502017 @default.
• W2103809350 countsByYear W21038093502018 @default.
• W2103809350 countsByYear W21038093502019 @default.
• W2103809350 countsByYear W21038093502021 @default.
• W2103809350 countsByYear W21038093502022 @default.
• W2103809350 crossrefType "journal-article" @default.

• next