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- W2103996971 abstract "Summary Increasing methionine in potato tubers is desirable, both to increase the availability of this limiting essential amino acid and to enhance the aroma of baked and fried potatoes. Previous attempts to elevate potato methionine content using transgenic approaches have focused on increasing methionine biosynthesis. Higher isoleucine accumulation in these transgenic tubers suggested that the potatoes compensate for increased methionine biosynthesis with enhanced catabolism via methionine gamma‐lyase ( MGL ), thereby producing 2‐ketybutyrate for isoleucine biosynthesis. In the current study, we show that potato St MGL 1 encodes a functional MGL in potato tubers. In planta silencing of St MGL 1 results in an increased methionine to isoleucine ratio in the free amino acid profile of potato tubers and, in some transgenic lines, elevated accumulation of free methionine. In both wild‐type and transgenic tubers, the ratio of methionine to isoleucine is negatively correlated with the level of St MGL 1 transcript. A three‐dimensional distribution of free amino acids in potato tubers is also described." @default.
- W2103996971 created "2016-06-24" @default.
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- W2103996971 date "2014-04-17" @default.
- W2103996971 modified "2023-09-30" @default.
- W2103996971 title "The catabolic enzyme methionine gamma-lyase limits methionine accumulation in potato tubers" @default.
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- W2103996971 doi "https://doi.org/10.1111/pbi.12191" @default.
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