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• W2104062578 abstract "Abstract Motivation: The genomic methylation analysis is useful to type bacteria that have a high number of expressed type II methyltransferases. Methyltransferases are usually committed to Restriction and Modification (R-M) systems, in which the restriction endonuclease imposes high pressure on the expression of the cognate methyltransferase that hinder R-M system loss. Conventional cluster methods do not reflect this tendency. An algorithm was developed for dendrogram construction reflecting the propensity for conservation of R-M Type II systems. Results: The new algorithm was applied to 52 Helicobacter pylori strains from different geographical regions and compared with conventional clustering methods. The algorithm works by first grouping strains that share a common minimum set of R-M systems and gradually adds strains according to the number of the R-M systems acquired. Dendrograms revealed a cluster of African strains, which suggest that R-M systems are present in H.pylori genome since its human host migrates from Africa. Availability: The software files are available at http://www.ff.ul.pt/paginas/jvitor/Bioinformatics/MCRM_algorithm.zip Contact: filipavale@fe.ucp.pt Supplementary information: Supplementary data are available at Bioinformatics online." @default.
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• W2104062578 date "2007-12-16" @default.
• W2104062578 modified "2023-09-27" @default.
• W2104062578 title "A new algorithm for cluster analysis of genomic methylation: the Helicobacter pylori case" @default.
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• W2104062578 doi "https://doi.org/10.1093/bioinformatics/btm621" @default.
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