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• W2104093664 abstract "The most common chromosomal abnormality observed in non-Hodgkin's lymphomas (NHL) involves the structural alteration of the q arm of chromosome 14. It is not always possible, however, to fully analyse such derivative chromosomes by Giemsa-banding. Therefore, we have applied the fluorescence in-situ hybridization (FISH) technique of chromosome painting to elucidate the origins of the der(14) chromosomes in 8 cases of NHL. In 2 NHL the der(14) appeared to be the product of the t(14;18)(q32;q21) translocation, but were not accompanied by the reciprocal der(18) chromosome. In 3 cases the breakpoint was at 14q32 but the translocated material appeared not to be from chromosome 18 and in 2 cases the breakpoint was centromeric to 14q32. One case with a t(14;18)(q32;q21) was also analysed as a control. Dual painting was carried out with paints for chromosome 14 and either chromosome 3, 8, 10, 11, 18 or 19. In the control and 2 other cases the translocated material was demonstrated to be from chromosome 18, in two cases it was from chromosome 3 and in 1 case there was an unusual insertion of chromosome 11 material. We were unable to identify the origins of the translocated material in 1 NHL and in the final case the apparent der(14) was demonstrated not to contain chromosome 14 material. These data demonstrated the utility of the FISH technique for analysing malignant cell karyotypes, and in particular indicated the potential of this approach for identifying cases containing putative NHL associated oncogenes that may have been translocated adjacent to the immunoglobulin locus at 14q32." @default.
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• W2104093664 date "1995-01-01" @default.
• W2104093664 modified "2023-09-25" @default.
• W2104093664 title "Analysis of 14q+ Derivative Chromosomes in Non-Hodgkin's Lymphomas by Fluorescence In-Situ Hybridization" @default.
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• W2104093664 doi "https://doi.org/10.3109/10428199509054761" @default.
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