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- W2104153716 abstract "A simple method, involving NAD+-Sepharose chromatography, was developed for the preparation of snake venom phosphodiesterase (EC 3.1.4.1) almost free from 5'-Nucleotidase (EC 3.1.3.5). Using an NAD+-Sepharose 4B column, phosphodiesterase was eluted in the unadsorbed fraction, whereas 5'nucleotidase was strongly adsorbed. The latter enzyme was desorbed when 0.2 M sodium bicarbonate buffer containing 1mM beta-NADH was used as a solvent. The affinity column could be used at least four times without any decrease of potency, and the method was applicable for the preparation of phosphodiesterase from the venoms of rattlesnake (Crotalus adamanteus) and Japanese mamushi (Agkistrodan halys blomhoffi)." @default.
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- W2104153716 date "1975-04-01" @default.
- W2104153716 modified "2023-09-27" @default.
- W2104153716 title "A Simple Method for Preparation of Snake Venom Phosphodiesterase almost Free from 5'-Nucleotidase1" @default.
- W2104153716 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a130790" @default.
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