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- W2104261281 abstract "Abstract The present study examined the effect of supplementation of the maturation medium with folic acid on the developmental competence of porcine oocytes as well as on the global DNA methylation of oocytes and histone acetylation in early developmental stage embryos. Supplementation of the maturation medium with 100 µM folic acid improved the development ratio of the oocytes to the blastocyst stage compared with those of oocytes that were cultured with either 0 or 10 µM folic acid. The global DNA methylation levels of the oocytes that matured with 100 µM folic acid were higher than those of oocytes that were cultured without folic acid, while the global DNA methylation levels of the oocytes decreased during maturation. Addition of either folic acid or 1.2 mM N-acetyl-cysteine (NAC) to the maturation medium significantly reduced the levels of reactive oxygen species in the oocytes. Supplementation of maturation medium with folic acid significantly enhanced the acetylation level of H4K8 and decreased th..." @default.
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- W2104261281 date "2013-10-01" @default.
- W2104261281 modified "2023-09-23" @default.
- W2104261281 title "Supplementation of Maturation Medium with Folic Acid Affects DNA Methylation of Porcine Oocytes and Histone Acetylation of Early Developmental Stage Embryos" @default.
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- W2104261281 doi "https://doi.org/10.1274/jmor.30.109" @default.
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