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• W2104416752 abstract "RNA interference (RNAi) leads to sequence specific knock-down of gene expression and has emerged as an important tool to analyse gene functions, pathway analysis and gene therapy. Although RNAi is a conserved cellular process involving common elements and factors, species-specific differences have been observed among different eukaryotes. Identification of components for RNAi pathway is pursued intensively and successful genome-wide screens have been performed for components of RNAi pathways in various organisms. Functional comparative genomics analysis offers evolutionary insight that forms basis of discoveries of novel RNAi-factors within related organisms. Keeping in view the academic and commercial utility of insect derived cell-line from Spodoptera frugiperda, we pursued the identification and functional analysis of components of RNAi-machinery of Sf21 cell-line using genome-wide application. The genome and transcriptome of Sf21 was assembled and annotated. In silico application of comparative genome analysis among insects allowed us to identify several RNAi factors in Sf21 line. The candidate RNAi factors from assembled genome were validated by knockdown analysis of candidate factors using the siRNA screens on the Sf21-gfp reporter cell-line. Forty two (42) potential factors were identified using the cell based assay. These include core RNAi elements including Dicer-2, Argonaute-1, Drosha, Aubergine and auxiliary modules like chromatin factors, RNA helicases, RNA processing module, signalling allied proteins and others. Phylogenetic analyses and domain architecture revealed that Spodoptera frugiperda homologs retained identity with Lepidoptera (Bombyx mori) or Coleoptera (Tribolium castaneum) sustaining an evolutionary conserved scaffold in post-transcriptional gene silencing paradigm within insects. The database of RNAi-factors generated by whole genome association survey offers comprehensive outlook about conservation as well as specific differences of the proteins of RNAi machinery. Understanding the interior involved in different phases of gene silencing also offers impending tool for RNAi-based applications." @default.
• W2104416752 created "2016-06-24" @default.
• W2104416752 creator A5052314553 @default.
• W2104416752 creator A5052932609 @default.
• W2104416752 creator A5052971052 @default.
• W2104416752 creator A5084679445 @default.
• W2104416752 creator A5084700817 @default.
• W2104416752 creator A5085144306 @default.
• W2104416752 date "2014-09-09" @default.
• W2104416752 modified "2023-10-09" @default.
• W2104416752 title "Genome wide screening of RNAi factors of Sf21 cells reveal several novel pathway associated proteins" @default.
• W2104416752 cites W1647075334 @default.
• W2104416752 cites W1731647989 @default.
• W2104416752 cites W1816295733 @default.
• W2104416752 cites W1916491745 @default.
• W2104416752 cites W1933947420 @default.
• W2104416752 cites W1967763256 @default.
• W2104416752 cites W1967959947 @default.
• W2104416752 cites W1969296875 @default.
• W2104416752 cites W1971549481 @default.
• W2104416752 cites W1975946390 @default.
• W2104416752 cites W1976342457 @default.
• W2104416752 cites W1978976893 @default.
• W2104416752 cites W1980269624 @default.
• W2104416752 cites W1982783106 @default.
• W2104416752 cites W1983871012 @default.
• W2104416752 cites W1984104677 @default.
• W2104416752 cites W1986034008 @default.
• W2104416752 cites W1989454102 @default.
• W2104416752 cites W1991737083 @default.
• W2104416752 cites W1995896257 @default.
• W2104416752 cites W1997570561 @default.
• W2104416752 cites W1997612057 @default.
• W2104416752 cites W2000210567 @default.
• W2104416752 cites W2004669233 @default.
• W2104416752 cites W2007091702 @default.
• W2104416752 cites W2007941919 @default.
• W2104416752 cites W2009644747 @default.
• W2104416752 cites W2009724373 @default.
• W2104416752 cites W2010404725 @default.
• W2104416752 cites W2011383767 @default.
• W2104416752 cites W2011816659 @default.
• W2104416752 cites W2017083894 @default.
• W2104416752 cites W2017184218 @default.
• W2104416752 cites W2017976049 @default.
• W2104416752 cites W2020120461 @default.
• W2104416752 cites W2020456835 @default.
• W2104416752 cites W2028466333 @default.
• W2104416752 cites W2031087599 @default.
• W2104416752 cites W2031283184 @default.
• W2104416752 cites W2032379393 @default.
• W2104416752 cites W2034293536 @default.
• W2104416752 cites W2035303830 @default.
• W2104416752 cites W2037863889 @default.
• W2104416752 cites W2037993016 @default.
• W2104416752 cites W2039245874 @default.
• W2104416752 cites W2039687496 @default.
• W2104416752 cites W2040234712 @default.
• W2104416752 cites W2040318443 @default.
• W2104416752 cites W2040665695 @default.
• W2104416752 cites W2041306541 @default.
• W2104416752 cites W2041594569 @default.
• W2104416752 cites W2045208896 @default.
• W2104416752 cites W2047256111 @default.
• W2104416752 cites W2049301633 @default.
• W2104416752 cites W2051252016 @default.
• W2104416752 cites W2053149533 @default.
• W2104416752 cites W2053250377 @default.
• W2104416752 cites W2065542901 @default.
• W2104416752 cites W2066077160 @default.
• W2104416752 cites W2066087020 @default.
• W2104416752 cites W2066511342 @default.
• W2104416752 cites W2067024190 @default.
• W2104416752 cites W2073718731 @default.
• W2104416752 cites W2074106269 @default.
• W2104416752 cites W2075433160 @default.
• W2104416752 cites W2077489356 @default.
• W2104416752 cites W2079902244 @default.
• W2104416752 cites W2083958499 @default.
• W2104416752 cites W2086227770 @default.
• W2104416752 cites W2087767674 @default.
• W2104416752 cites W2090312783 @default.
• W2104416752 cites W2094395550 @default.
• W2104416752 cites W2095870428 @default.
• W2104416752 cites W2097676237 @default.
• W2104416752 cites W2104582743 @default.
• W2104416752 cites W2111009710 @default.
• W2104416752 cites W2111092051 @default.
• W2104416752 cites W2111366309 @default.
• W2104416752 cites W2111578933 @default.
• W2104416752 cites W2112572048 @default.
• W2104416752 cites W2114582526 @default.
• W2104416752 cites W2115116283 @default.
• W2104416752 cites W2115829042 @default.
• W2104416752 cites W2117991391 @default.
• W2104416752 cites W2119015197 @default.
• W2104416752 cites W2123957786 @default.
• W2104416752 cites W2124791602 @default.
• W2104416752 cites W2126417923 @default.
• W2104416752 cites W2128882718 @default.

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