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- W2104422107 endingPage "8988" @default.
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- W2104422107 abstract "The nucleocapsid protein (NC) of human immunodeficiency virus type 1 (HIV-1) has two zinc fingers, each containing the invariant metal ion binding residues CCHC. Recent reports indicate that mutations in the CCHC motifs are deleterious for reverse transcription in vivo. To identify reverse transcriptase (RT) reactions affected by such changes, we have probed zinc finger functions in NC-dependent RT-catalyzed HIV-1 minus- and plus-strand transfer model systems. Our approach was to examine the activities of wild-type NC and a mutant in which all six cysteine residues were replaced by serine (SSHS NC); this mutation severely disrupts zinc coordination. We find that the zinc fingers contribute to the role of NC in complete tRNA primer removal from minus-strand DNA during plus-strand transfer. Annealing of the primer binding site sequences in plus-strand strong-stop DNA [(+) SSDNA] to its complement in minus-strand acceptor DNA is not dependent on NC zinc fingers. In contrast, the rate of annealing of the complementary R regions in (-) SSDNA and 3' viral RNA during minus-strand transfer is approximately eightfold lower when SSHS NC is used in place of wild-type NC. Moreover, unlike wild-type NC, SSHS NC has only a small stimulatory effect on minus-strand transfer and is essentially unable to block TAR-induced self-priming from (-) SSDNA. Our results strongly suggest that NC zinc finger structures are needed to unfold highly structured RNA and DNA strand transfer intermediates. Thus, it appears that in these cases, zinc finger interactions are important components of NC nucleic acid chaperone activity." @default.
- W2104422107 created "2016-06-24" @default.
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- W2104422107 creator A5078747233 @default.
- W2104422107 date "2000-10-01" @default.
- W2104422107 modified "2023-10-16" @default.
- W2104422107 title "Zinc Finger Structures in the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Facilitate Efficient Minus- and Plus-Strand Transfer" @default.
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- W2104422107 doi "https://doi.org/10.1128/jvi.74.19.8980-8988.2000" @default.
- W2104422107 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/102094" @default.
- W2104422107 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10982342" @default.
- W2104422107 hasPublicationYear "2000" @default.