FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2104449484> ?p ?o ?g. }

• W2104449484 endingPage "1203" @default.
• W2104449484 startingPage "1190" @default.
• W2104449484 abstract "Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) is an anchorless, multifunctional protein displayed on the surface of several fungi and Gram-positive pathogens, which contributes to their adhesion and virulence. To date a role for extracellular GAPDH in the pathogenesis of Gram-negative bacteria has not been described. The aim of this study was to analyze the extracellular localization of GAPDH in enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains and to examine its interaction with host components that could be related to the infection mechanism. Recombinant E. coli GAPDH was purified and polyclonal antibodies were obtained. Western blotting and immunoelectron microscopy showed that GAPDH is located on the bacterial surface and released to the culture medium of EHEC and EPEC strains. GAPDH export in these Gram-negative pathogens depends on the external medium, is not mediated by vesicles and leads to an extracellular active enzyme. Non-pathogenic E. coli strains do not secrete GAPDH. Two-dimensional electrophoresis analysis showed that in E. coli GAPDH is present at least in two major forms with different isoelectric points. Of these forms, the more basic is secreted. Purified GAPDH was found to bind human plasminogen and fibrinogen in Far-Western blot and ELISA-based assays. In addition, GAPDH remained associated with colonic Caco-2 epithelial cells after adhesion of EHEC or EPEC. These observations indicate that exported GAPDH may act as a virulence factor which could contribute to EHEC and EPEC pathogenesis. This is the first description of an extracellular localization for this enzyme, with a function other than its glycolytic role in Gram-negative pathogens." @default.
• W2104449484 created "2016-06-24" @default.
• W2104449484 creator A5008998335 @default.
• W2104449484 creator A5029570457 @default.
• W2104449484 creator A5029648082 @default.
• W2104449484 creator A5051703756 @default.
• W2104449484 creator A5060303204 @default.
• W2104449484 creator A5083397885 @default.
• W2104449484 creator A5087258620 @default.
• W2104449484 date "2007-01-01" @default.
• W2104449484 modified "2023-10-18" @default.
• W2104449484 title "Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: Interaction of the extracellular enzyme with human plasminogen and fibrinogen" @default.
• W2104449484 cites W1555791296 @default.
• W2104449484 cites W1578827617 @default.
• W2104449484 cites W1764369073 @default.
• W2104449484 cites W1882854473 @default.
• W2104449484 cites W1907149689 @default.
• W2104449484 cites W1912062656 @default.
• W2104449484 cites W1979615338 @default.
• W2104449484 cites W1988999243 @default.
• W2104449484 cites W1995428252 @default.
• W2104449484 cites W1997880257 @default.
• W2104449484 cites W2003688768 @default.
• W2104449484 cites W2008854080 @default.
• W2104449484 cites W2012090721 @default.
• W2104449484 cites W2013531699 @default.
• W2104449484 cites W2019615869 @default.
• W2104449484 cites W2032802468 @default.
• W2104449484 cites W2034911512 @default.
• W2104449484 cites W2035295085 @default.
• W2104449484 cites W2071640711 @default.
• W2104449484 cites W2075358965 @default.
• W2104449484 cites W2076698269 @default.
• W2104449484 cites W2077691179 @default.
• W2104449484 cites W2083100284 @default.
• W2104449484 cites W2090611703 @default.
• W2104449484 cites W2100837269 @default.
• W2104449484 cites W2103263040 @default.
• W2104449484 cites W2120556801 @default.
• W2104449484 cites W2140371210 @default.
• W2104449484 cites W2142307902 @default.
• W2104449484 cites W2147203214 @default.
• W2104449484 cites W2147399606 @default.
• W2104449484 cites W2156810094 @default.
• W2104449484 cites W2167152465 @default.
• W2104449484 cites W2169011707 @default.
• W2104449484 cites W2169191160 @default.
• W2104449484 doi "https://doi.org/10.1016/j.biocel.2007.03.008" @default.
• W2104449484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17449317" @default.
• W2104449484 hasPublicationYear "2007" @default.
• W2104449484 type Work @default.
• W2104449484 sameAs 2104449484 @default.
• W2104449484 citedByCount "132" @default.
• W2104449484 countsByYear W21044494842012 @default.
• W2104449484 countsByYear W21044494842013 @default.
• W2104449484 countsByYear W21044494842014 @default.
• W2104449484 countsByYear W21044494842015 @default.
• W2104449484 countsByYear W21044494842016 @default.
• W2104449484 countsByYear W21044494842017 @default.
• W2104449484 countsByYear W21044494842018 @default.
• W2104449484 countsByYear W21044494842019 @default.
• W2104449484 countsByYear W21044494842020 @default.
• W2104449484 countsByYear W21044494842021 @default.
• W2104449484 countsByYear W21044494842022 @default.
• W2104449484 countsByYear W21044494842023 @default.
• W2104449484 crossrefType "journal-article" @default.
• W2104449484 hasAuthorship W2104449484A5008998335 @default.
• W2104449484 hasAuthorship W2104449484A5029570457 @default.
• W2104449484 hasAuthorship W2104449484A5029648082 @default.
• W2104449484 hasAuthorship W2104449484A5051703756 @default.
• W2104449484 hasAuthorship W2104449484A5060303204 @default.
• W2104449484 hasAuthorship W2104449484A5083397885 @default.
• W2104449484 hasAuthorship W2104449484A5087258620 @default.
• W2104449484 hasConcept C104317684 @default.
• W2104449484 hasConcept C134792803 @default.
• W2104449484 hasConcept C146587185 @default.
• W2104449484 hasConcept C153911025 @default.
• W2104449484 hasConcept C181199279 @default.
• W2104449484 hasConcept C2776317432 @default.
• W2104449484 hasConcept C2776415932 @default.
• W2104449484 hasConcept C2781090273 @default.
• W2104449484 hasConcept C28406088 @default.
• W2104449484 hasConcept C2910478007 @default.
• W2104449484 hasConcept C547475151 @default.
• W2104449484 hasConcept C55493867 @default.
• W2104449484 hasConcept C60987743 @default.
• W2104449484 hasConcept C86803240 @default.
• W2104449484 hasConcept C89423630 @default.
• W2104449484 hasConceptScore W2104449484C104317684 @default.
• W2104449484 hasConceptScore W2104449484C134792803 @default.
• W2104449484 hasConceptScore W2104449484C146587185 @default.
• W2104449484 hasConceptScore W2104449484C153911025 @default.
• W2104449484 hasConceptScore W2104449484C181199279 @default.
• W2104449484 hasConceptScore W2104449484C2776317432 @default.
• W2104449484 hasConceptScore W2104449484C2776415932 @default.
• W2104449484 hasConceptScore W2104449484C2781090273 @default.
• W2104449484 hasConceptScore W2104449484C28406088 @default.
• W2104449484 hasConceptScore W2104449484C2910478007 @default.

• next