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- W2104457107 abstract "Bladder contraction is mediated by a number of intracellular biochemical reactions including glycolysis, mitochondrial respiration, phosphocreatine kinase, and various ATPases. In order to understand how alterations in intracellular metabolism can affect bladder function (or how alterations in bladder function can affect intracellular metabolism), we must first understand and describe normal bladder metabolism. Using the rabbit as a model for bladder function, the present study compares the intracellular concentrations of a variety of important compounds between the bladder base and body. The intracellular concentrations of phosphocreatine (PC), creatine, ATP, ADP, and AMP were determined in samples of rabbit urinary bladder body and base. The values for PC and creatine were significantly lower in the bladder base than in the bladder body. Adenine nucleotide concentrations were similar in the two sections. The concentrations of NAD + NADH, NADP, CTP, CDP, CMP, GTP, GDP, GMP, and UTP, UDP, UMP were also measured. Of these compounds, the concentrations of GTP and UTP in the bladder body were nearly double those in the bladder base. The intracellular concentrations of the other tissue constituents were similar in the two bladder sections. These studies demonstrate that the bladder is not biochemically homogeneous but that the bladder base and body differ not only functionally but also metabolically. Experiments in which strips of bladder body were incubated in vitro in oxygenated Tyrode's solution containing glucose showed that the tissue concentrations of PC, ATP, GTP, and UTP decreased substantially following 30 min of incubation, with no further decrease during a subsequent 60-min incubation." @default.
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- W2104457107 date "1989-01-01" @default.
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- W2104457107 title "Biochemical characterization of the rabbit urinary bladder: II. Intracellular concentration of nucleotides" @default.
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- W2104457107 doi "https://doi.org/10.1002/nau.1930080107" @default.
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