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• W2104492942 abstract "Cytomegalovirus (CMV) infection is the most common cause of congenital infection. Whereas CMV PCR has replaced viral culture and antigen detection in immunocompromised patients because of higher sensitivity, viral culture of neonatal urine is still referred to as the gold standard in the diagnosis of congenital CMV infection. To compare real-time CMV PCR with shell vial culture on urine in the diagnosis of congenital CMV, in a multicenter design. A series of neonatal urines (n = 340), received for congenital CMV diagnostics and routinely assessed with shell vial CMV culture, was retrospectively tested by real-time CMV PCR. The proportion of newborns found to be congenitally infected by real-time CMV PCR was 8.2% (28/340, 95%CI 5.6–11.8%), and 7.4% (25/340, 95%CI 4.9–10.8%) by rapid culture. When considering rapid culture as reference, real-time PCR was highly sensitive (100%), whereas sensitivity of rapid culture was 89.3% when considering real-time PCR as reference. Our results, supported by analytical and clinical data on CMV DNA detection in neonatal urine, suggest enhanced sensitivity of recent PCR techniques when compared to viral culture. There is considerable rationale to favor real-time CMV PCR as a gold standard in the diagnosis of congenital CMV infection. A large-scale study combining both laboratory and clinical data is required to determine the exact time frame for sampling of neonatal urine when using real-time PCR." @default.
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• W2104492942 date "2012-02-01" @default.
• W2104492942 modified "2023-10-06" @default.
• W2104492942 title "Real-time PCR versus viral culture on urine as a gold standard in the diagnosis of congenital cytomegalovirus infection" @default.
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• W2104492942 doi "https://doi.org/10.1016/j.jcv.2011.11.006" @default.
• W2104492942 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22177273" @default.
• W2104492942 hasPublicationYear "2012" @default.
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