FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2104571489> ?p ?o ?g. }

• W2104571489 abstract "Increasing rates of antimicrobial resistance among uropathogens led, among other efforts, to the application of subtractive reverse vaccinology for the identification of antigens present in extraintestinal pathogenic E. coli (ExPEC) strains but absent or variable in non-pathogenic strains, in a quest for a broadly protective Escherichia coli vaccine. The protein coded by locus c5321 from CFT073 E. coli was identified as one of nine potential vaccine candidates against ExPEC and was able to confer protection with an efficacy of 33% in a mouse model of sepsis. c5321 (known also as EsiB) lacks functional annotation and structurally belongs to the Sel1-like repeat (SLR) family. Herein, as part of the general characterization of this potential antigen, we have focused on its structural properties.We report the 1.74 Å-resolution crystal structure of c5321 from CFT073 E. coli determined by Se-Met SAD phasing. The structure is composed of 11 SLR units in a topological organisation that highly resembles that found in HcpC from Helicobacter pylori, with the main difference residing in how the super-helical fold is stabilised. The stabilising effect of disulfide bridges in HcpC is replaced in c5321 by a strengthening of the inter-repeat hydrophobic core. A metal-ion binding site, uncharacteristic of SLR proteins, is detected between SLR units 3 and 4 in the region of the inter-repeat hydrophobic core. Crystal contacts are observed between the C-terminal tail of one molecule and the C-terminal amphipathic groove of a neighbouring one, resembling interactions between ligand and proteins containing tetratricopeptide-like repeats.The structure of antigen c5321 presents a mode of stabilization of the SLR fold different from that observed in close homologs of known structure. The location of the metal-ion binding site and the observed crystal contacts suggest a potential role in regulation of conformational flexibility and interaction with yet unidentified target proteins, respectively. These findings open new perspectives in both antigen design and for the identification of a functional role for this protective antigen." @default.
• W2104571489 created "2016-06-24" @default.
• W2104571489 creator A5003132365 @default.
• W2104571489 creator A5004223415 @default.
• W2104571489 creator A5027747641 @default.
• W2104571489 creator A5036439202 @default.
• W2104571489 creator A5037064815 @default.
• W2104571489 creator A5039996592 @default.
• W2104571489 creator A5053099063 @default.
• W2104571489 creator A5072941072 @default.
• W2104571489 creator A5076914476 @default.
• W2104571489 creator A5088452240 @default.
• W2104571489 creator A5089690933 @default.
• W2104571489 creator A5025344018 @default.
• W2104571489 date "2013-10-07" @default.
• W2104571489 modified "2023-09-26" @default.
• W2104571489 title "Crystal structure of c5321: a protective antigen present in uropathogenic Escherichia coli strains displaying an SLR fold" @default.
• W2104571489 cites W1849202109 @default.
• W2104571489 cites W1972223538 @default.
• W2104571489 cites W1972583509 @default.
• W2104571489 cites W1977922844 @default.
• W2104571489 cites W1985648234 @default.
• W2104571489 cites W1986191025 @default.
• W2104571489 cites W1988642730 @default.
• W2104571489 cites W1997087248 @default.
• W2104571489 cites W2024269030 @default.
• W2104571489 cites W2042911807 @default.
• W2104571489 cites W2049631853 @default.
• W2104571489 cites W2055122966 @default.
• W2104571489 cites W2055382743 @default.
• W2104571489 cites W2056550440 @default.
• W2104571489 cites W2060906834 @default.
• W2104571489 cites W2067391857 @default.
• W2104571489 cites W2069871720 @default.
• W2104571489 cites W2074131174 @default.
• W2104571489 cites W2081415771 @default.
• W2104571489 cites W2091317572 @default.
• W2104571489 cites W2091979095 @default.
• W2104571489 cites W2094201044 @default.
• W2104571489 cites W2105902442 @default.
• W2104571489 cites W2112695987 @default.
• W2104571489 cites W2117727476 @default.
• W2104571489 cites W2121069774 @default.
• W2104571489 cites W2124672930 @default.
• W2104571489 cites W2136296923 @default.
• W2104571489 cites W2137353223 @default.
• W2104571489 cites W2143815085 @default.
• W2104571489 cites W2153139855 @default.
• W2104571489 cites W2160545383 @default.
• W2104571489 cites W2164836215 @default.
• W2104571489 cites W2180229411 @default.
• W2104571489 cites W4320301318 @default.
• W2104571489 doi "https://doi.org/10.1186/1472-6807-13-19" @default.
• W2104571489 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3851747" @default.
• W2104571489 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24099525" @default.
• W2104571489 hasPublicationYear "2013" @default.
• W2104571489 type Work @default.
• W2104571489 sameAs 2104571489 @default.
• W2104571489 citedByCount "15" @default.
• W2104571489 countsByYear W21045714892014 @default.
• W2104571489 countsByYear W21045714892016 @default.
• W2104571489 countsByYear W21045714892018 @default.
• W2104571489 countsByYear W21045714892019 @default.
• W2104571489 countsByYear W21045714892020 @default.
• W2104571489 countsByYear W21045714892023 @default.
• W2104571489 crossrefType "journal-article" @default.
• W2104571489 hasAuthorship W2104571489A5003132365 @default.
• W2104571489 hasAuthorship W2104571489A5004223415 @default.
• W2104571489 hasAuthorship W2104571489A5025344018 @default.
• W2104571489 hasAuthorship W2104571489A5027747641 @default.
• W2104571489 hasAuthorship W2104571489A5036439202 @default.
• W2104571489 hasAuthorship W2104571489A5037064815 @default.
• W2104571489 hasAuthorship W2104571489A5039996592 @default.
• W2104571489 hasAuthorship W2104571489A5053099063 @default.
• W2104571489 hasAuthorship W2104571489A5072941072 @default.
• W2104571489 hasAuthorship W2104571489A5076914476 @default.
• W2104571489 hasAuthorship W2104571489A5088452240 @default.
• W2104571489 hasAuthorship W2104571489A5089690933 @default.
• W2104571489 hasBestOaLocation W21045714891 @default.
• W2104571489 hasConcept C104317684 @default.
• W2104571489 hasConcept C147483822 @default.
• W2104571489 hasConcept C167625842 @default.
• W2104571489 hasConcept C185592680 @default.
• W2104571489 hasConcept C54355233 @default.
• W2104571489 hasConcept C547475151 @default.
• W2104571489 hasConcept C55493867 @default.
• W2104571489 hasConcept C65556437 @default.
• W2104571489 hasConcept C86803240 @default.
• W2104571489 hasConcept C89423630 @default.
• W2104571489 hasConceptScore W2104571489C104317684 @default.
• W2104571489 hasConceptScore W2104571489C147483822 @default.
• W2104571489 hasConceptScore W2104571489C167625842 @default.
• W2104571489 hasConceptScore W2104571489C185592680 @default.
• W2104571489 hasConceptScore W2104571489C54355233 @default.
• W2104571489 hasConceptScore W2104571489C547475151 @default.
• W2104571489 hasConceptScore W2104571489C55493867 @default.
• W2104571489 hasConceptScore W2104571489C65556437 @default.
• W2104571489 hasConceptScore W2104571489C86803240 @default.
• W2104571489 hasConceptScore W2104571489C89423630 @default.
• W2104571489 hasIssue "1" @default.

• next