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- W2104614171 endingPage "1926" @default.
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- W2104614171 abstract "Tumors express more than a single angiogenic growth factor. To investigate the relative impact of fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factor (VEGF) on tumor growth and neovascularization, we generated tumor cell transfectants differing for VEGF and/or FGF-2 expression. Human endometrial adenocarcinoma HEC-1-B-derived Tet-FGF-2 cells that express FGF-2 under the control of the tetracycline-responsive promoter (Tet-off system) were further transfected with a VEGF(121) anti-sense (AS-VEGF) cDNA. Next, Tet-FGF-2 and AS-VEGF/Tet-FGF-2 cells were transplanted subcutaneously in nude mice that received tetracycline or not in the drinking water. Simultaneous expression of FGF-2 and VEGF in Tet-FGF-2 cells resulted in fast-growing lesions characterized by high blood vessel density, patency and permeability, and limited necrosis. Blood vessels were highly heterogeneous in size and frequently associated with pericytes. Inhibition of FGF-2 production by tetracycline caused a significant decrease in tumor burden paralleled by a decrease in blood vessel density and size. AS-VEGF expression resulted in a similar reduction in blood vessel density associated with a significant decrease in pericyte organization, vascular patency, and permeability. The consequent decrease in tumor burden was paralleled by increased tumor hypoxia and necrosis. A limited additional inhibitory effect was exerted by simultaneous down-regulation of FGF-2 and VEGF expression. These findings demonstrate that FGF-2 and VEGF stimulate vascularization synergistically but with distinctive effects on vessel functionality and tumor survival. Blockade of either one of the two growth factors results in a decrease in blood vessel density and, consequently, in tumor burden. However, inhibition of the expression of VEGF, but not of FGF-2, affects also vessel maturation and functionality, leading to tumor hypoxia and necrosis. Our experimental model represents an unique tool to investigate anti-neoplastic therapies in different angiogenic environments." @default.
- W2104614171 created "2016-06-24" @default.
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- W2104614171 date "2003-06-01" @default.
- W2104614171 modified "2023-10-17" @default.
- W2104614171 title "Distinct Role of Fibroblast Growth Factor-2 and Vascular Endothelial Growth Factor on Tumor Growth and Angiogenesis" @default.
- W2104614171 cites W122369139 @default.
- W2104614171 cites W12613825 @default.
- W2104614171 cites W1479988956 @default.
- W2104614171 cites W1487434453 @default.
- W2104614171 cites W1492332150 @default.
- W2104614171 cites W1504359409 @default.
- W2104614171 cites W1563686754 @default.
- W2104614171 cites W1573363574 @default.
- W2104614171 cites W1573798878 @default.
- W2104614171 cites W1578283200 @default.
- W2104614171 cites W1600542667 @default.
- W2104614171 cites W1603230122 @default.
- W2104614171 cites W1604140953 @default.
- W2104614171 cites W1611565221 @default.
- W2104614171 cites W1636670860 @default.
- W2104614171 cites W1665704747 @default.
- W2104614171 cites W1673123614 @default.
- W2104614171 cites W1714545534 @default.
- W2104614171 cites W1756949381 @default.
- W2104614171 cites W1783580437 @default.
- W2104614171 cites W1918337600 @default.
- W2104614171 cites W1969328947 @default.
- W2104614171 cites W198059592 @default.
- W2104614171 cites W1982509041 @default.
- W2104614171 cites W1984690727 @default.
- W2104614171 cites W1985712533 @default.
- W2104614171 cites W1985737377 @default.
- W2104614171 cites W1987873158 @default.
- W2104614171 cites W1990497183 @default.
- W2104614171 cites W1991050551 @default.
- W2104614171 cites W1991472045 @default.
- W2104614171 cites W1993866602 @default.
- W2104614171 cites W1994131389 @default.
- W2104614171 cites W2000000541 @default.
- W2104614171 cites W2000892993 @default.
- W2104614171 cites W2001142764 @default.
- W2104614171 cites W2012255417 @default.
- W2104614171 cites W2017041417 @default.
- W2104614171 cites W2025036947 @default.
- W2104614171 cites W2029883816 @default.
- W2104614171 cites W2037141117 @default.
- W2104614171 cites W2039176290 @default.
- W2104614171 cites W2043850275 @default.
- W2104614171 cites W2046432580 @default.
- W2104614171 cites W2049042095 @default.
- W2104614171 cites W2049408374 @default.
- W2104614171 cites W2054445563 @default.
- W2104614171 cites W2060100969 @default.
- W2104614171 cites W2061230824 @default.
- W2104614171 cites W2064419187 @default.
- W2104614171 cites W2064624801 @default.
- W2104614171 cites W2067055586 @default.
- W2104614171 cites W2069698188 @default.
- W2104614171 cites W2077688090 @default.
- W2104614171 cites W2081440269 @default.
- W2104614171 cites W2082512712 @default.
- W2104614171 cites W2085061661 @default.
- W2104614171 cites W2093498435 @default.
- W2104614171 cites W2093915058 @default.
- W2104614171 cites W2099722352 @default.
- W2104614171 cites W2104114689 @default.
- W2104614171 cites W2111266343 @default.
- W2104614171 cites W2119308535 @default.
- W2104614171 cites W2130219349 @default.
- W2104614171 cites W2132520492 @default.
- W2104614171 cites W2146564308 @default.
- W2104614171 cites W2156113506 @default.
- W2104614171 cites W2157892961 @default.
- W2104614171 cites W2159789252 @default.
- W2104614171 cites W2159957159 @default.
- W2104614171 cites W2161562135 @default.
- W2104614171 cites W2161714684 @default.
- W2104614171 cites W2162472165 @default.
- W2104614171 cites W2169199162 @default.
- W2104614171 cites W2320669141 @default.
- W2104614171 cites W2332974012 @default.
- W2104614171 cites W2397530495 @default.
- W2104614171 cites W2398880988 @default.
- W2104614171 cites W2416881637 @default.
- W2104614171 cites W2474616344 @default.
- W2104614171 cites W2580449060 @default.
- W2104614171 cites W32167704 @default.
- W2104614171 cites W4247664461 @default.
- W2104614171 cites W4255042798 @default.
- W2104614171 cites W1592012606 @default.