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- W2104663981 abstract "Nuclear mRNAs in trypanosomatids are generated by trans-splicing. Although trans-splicing resembles cis-splicing in many ways and most of the U RNA participants have been characterized, relatively few involved proteins have been identified. Herein, we employed a yeast three-hybrid system to identify a protein, XB1, which binds to the Trypanosoma cruzi SL RNA. XB1 is a approximately 45 kDa protein which is homologous to the essential pre-mRNA-splicing factor PRP31p from Saccharomyces cerevisiae. Gel shift assays and UV cross-linking experiments with recombinant XB1 confirmed that this T. cruzi protein binds the SL RNA in vitro. The binding site of XB1 on the SL RNA was mapped to stem-loop II by deletion of the SL RNA 'bait' in the three-hybrid system. Finally, UV cross-linking SL RNA with S100 extract indicated native XB1 protein and SL RNA interaction in T. cruzi extract." @default.
- W2104663981 created "2016-06-24" @default.
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- W2104663981 date "2001-01-01" @default.
- W2104663981 modified "2023-10-17" @default.
- W2104663981 title "Identification of a spliced leader RNA binding protein from Trypanosoma cruzi" @default.
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- W2104663981 doi "https://doi.org/10.1016/s0166-6851(00)00341-8" @default.
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