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• W2104711227 abstract "For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination." @default.
• W2104711227 created "2016-06-24" @default.
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• W2104711227 date "2009-01-01" @default.
• W2104711227 modified "2023-09-25" @default.
• W2104711227 title "MIG (CXCL9) is a more sensitive measure than IFN-γ of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines" @default.
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• W2104711227 doi "https://doi.org/10.1016/j.jim.2008.09.021" @default.
• W2104711227 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2648876" @default.
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