Matches in SemOpenAlex for { <https://semopenalex.org/work/W2104741425> ?p ?o ?g. }
- W2104741425 endingPage "7890" @default.
- W2104741425 startingPage "7879" @default.
- W2104741425 abstract "Nonoverlapping deletions that eliminated the 5' (HIV-1US/603del), middle (HIV-1U5/206del), and 3' (HIV-1U5/604del) thirds of the U5 region of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) were studied for their effects on virus replication (transient transfection of HeLa cells) and infectivity (T-cell lines and peripheral blood mononuclear cells). All three mutants exhibited a wild-type phenotype in directing the production and release of virus particles from transfected HeLa cells. In infectivity assays, HIV-1U5/206del was usually indistinguishable from wild-type virus whereas HIV-1U%/603del was unable to infect human peripheral blood mononuclear cells or MT4 and CEM cells. Investigations of HIV-1U5/603del particles revealed a packaging defect resulting in a 10-fold reduction of encapsidated genomic RNA. The HIV-1U5/604del mutant either was noninfectious or exhibited delayed infection kinetics, depending on the cell type and multiplicity of infection. Quantitative competitive PCR indicated that HIV-1U5/604del synthesized normal amounts of viral DNA in newly infected cells. During the course of a long-term infectivity assay, a revertant of the HIV-1U5/604del mutant that displayed rapid infection kinetics emerged. Nucleotide sequence analysis indicated that the original 26-nucleotide deletion present in HIV-1U5/604del had been extended an additional 19 nucleotides in the revertant virus. Characterization of the HIV-1U5/604del mutant LTR in in vitro integration reactions revealed defective 3' processing and strand transfer activities that were partially restored when the revertant LTR substrate was used, suggesting that the reversion corrected a similar defect in the mutant virus." @default.
- W2104741425 created "2016-06-24" @default.
- W2104741425 creator A5020623452 @default.
- W2104741425 creator A5049119347 @default.
- W2104741425 creator A5055244754 @default.
- W2104741425 creator A5058005825 @default.
- W2104741425 creator A5058843647 @default.
- W2104741425 creator A5062067515 @default.
- W2104741425 creator A5082700686 @default.
- W2104741425 creator A5089862290 @default.
- W2104741425 date "1994-12-01" @default.
- W2104741425 modified "2023-10-15" @default.
- W2104741425 title "An integration-defective U5 deletion mutant of human immunodeficiency virus type 1 reverts by eliminating additional long terminal repeat sequences" @default.
- W2104741425 cites W1488279264 @default.
- W2104741425 cites W1521884339 @default.
- W2104741425 cites W1524005178 @default.
- W2104741425 cites W1527352542 @default.
- W2104741425 cites W1535147929 @default.
- W2104741425 cites W1546979502 @default.
- W2104741425 cites W1556458994 @default.
- W2104741425 cites W1557952727 @default.
- W2104741425 cites W1562851580 @default.
- W2104741425 cites W1564738436 @default.
- W2104741425 cites W1574302018 @default.
- W2104741425 cites W1590989003 @default.
- W2104741425 cites W1599509724 @default.
- W2104741425 cites W1605873417 @default.
- W2104741425 cites W1613477799 @default.
- W2104741425 cites W1619075339 @default.
- W2104741425 cites W1621406154 @default.
- W2104741425 cites W1629201894 @default.
- W2104741425 cites W1630984310 @default.
- W2104741425 cites W1655088763 @default.
- W2104741425 cites W1659719610 @default.
- W2104741425 cites W1685289055 @default.
- W2104741425 cites W1687759833 @default.
- W2104741425 cites W1806273468 @default.
- W2104741425 cites W1807924095 @default.
- W2104741425 cites W1827240581 @default.
- W2104741425 cites W1875262379 @default.
- W2104741425 cites W1880169023 @default.
- W2104741425 cites W1917655123 @default.
- W2104741425 cites W1920597859 @default.
- W2104741425 cites W1961106101 @default.
- W2104741425 cites W1965528983 @default.
- W2104741425 cites W1966815449 @default.
- W2104741425 cites W1967446284 @default.
- W2104741425 cites W1971994381 @default.
- W2104741425 cites W1984125372 @default.
- W2104741425 cites W1991446221 @default.
- W2104741425 cites W2002907404 @default.
- W2104741425 cites W2004291887 @default.
- W2104741425 cites W2007973055 @default.
- W2104741425 cites W2014568295 @default.
- W2104741425 cites W2015326137 @default.
- W2104741425 cites W2021650808 @default.
- W2104741425 cites W2025260589 @default.
- W2104741425 cites W2036931150 @default.
- W2104741425 cites W2041632561 @default.
- W2104741425 cites W2044253644 @default.
- W2104741425 cites W2054395379 @default.
- W2104741425 cites W2065979160 @default.
- W2104741425 cites W2089882643 @default.
- W2104741425 cites W2094657382 @default.
- W2104741425 cites W2095816327 @default.
- W2104741425 cites W2098272959 @default.
- W2104741425 cites W2101779661 @default.
- W2104741425 cites W2105648514 @default.
- W2104741425 cites W2138270253 @default.
- W2104741425 cites W2139018692 @default.
- W2104741425 cites W2144818221 @default.
- W2104741425 cites W2163591430 @default.
- W2104741425 cites W2163604069 @default.
- W2104741425 cites W2336149973 @default.
- W2104741425 doi "https://doi.org/10.1128/jvi.68.12.7879-7890.1994" @default.
- W2104741425 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/237250" @default.
- W2104741425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7966578" @default.
- W2104741425 hasPublicationYear "1994" @default.
- W2104741425 type Work @default.
- W2104741425 sameAs 2104741425 @default.
- W2104741425 citedByCount "60" @default.
- W2104741425 countsByYear W21047414252012 @default.
- W2104741425 countsByYear W21047414252013 @default.
- W2104741425 countsByYear W21047414252015 @default.
- W2104741425 countsByYear W21047414252017 @default.
- W2104741425 countsByYear W21047414252019 @default.
- W2104741425 crossrefType "journal-article" @default.
- W2104741425 hasAuthorship W2104741425A5020623452 @default.
- W2104741425 hasAuthorship W2104741425A5049119347 @default.
- W2104741425 hasAuthorship W2104741425A5055244754 @default.
- W2104741425 hasAuthorship W2104741425A5058005825 @default.
- W2104741425 hasAuthorship W2104741425A5058843647 @default.
- W2104741425 hasAuthorship W2104741425A5062067515 @default.
- W2104741425 hasAuthorship W2104741425A5082700686 @default.
- W2104741425 hasAuthorship W2104741425A5089862290 @default.
- W2104741425 hasBestOaLocation W21047414251 @default.
- W2104741425 hasConcept C104317684 @default.
- W2104741425 hasConcept C106358424 @default.