FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2104813426> ?p ?o ?g. }

• W2104813426 abstract "Bacillus megaterium expressing a variant of the green fluorescent protein (GFP) was used to investigate the influence of gradients in dissolved oxygen (DO) and fructose concentration on biomass and product formation. For this purpose, several fed-batch experiments with DO as feed control parameter were performed using suboptimal and optimal adjusted control parameters. In a first approach, suboptimal DO-feeding was reached by varying randomly the controller output between 10 and 20% of the maximal pumping capacity. This led to fluctuations in DO between 20 and 80% and to substrate gradients around 3 g/L. GFP formation thereby was decreased. In a second approach, an optimal feeding profile was realized using a PI-control with a constant controller output of 20% was used. Thereby no gradients in substrate concentration, but low-amplitude and high-frequency oscillations in the DO concentration were obtained, leading to increased GFP formation. In a final experimental approach, large-scale conditions were emulated in a two-compartment scale-down system consisting of a 3.7-L stirred tank reactor and a 0.7-L non-stirred vessel. DO-based feeding was applied with the measurement in the 3.7-L bioreactor and feeding in the small vessel. This setup led to a fairly constant gradient of 20% between both vessels, which also resulted in a decreased GFP productivity." @default.
• W2104813426 created "2016-06-24" @default.
• W2104813426 creator A5005359953 @default.
• W2104813426 creator A5031893561 @default.
• W2104813426 creator A5072037290 @default.
• W2104813426 creator A5078624374 @default.
• W2104813426 date "2011-08-01" @default.
• W2104813426 modified "2023-09-26" @default.
• W2104813426 title "Influence of fructose and oxygen gradients on fed-batch recombinant protein production using Bacillus megaterium" @default.
• W2104813426 cites W164573055 @default.
• W2104813426 cites W1977445290 @default.
• W2104813426 cites W1979610409 @default.
• W2104813426 cites W1980740090 @default.
• W2104813426 cites W1988982796 @default.
• W2104813426 cites W1999982222 @default.
• W2104813426 cites W2012890940 @default.
• W2104813426 cites W2017287753 @default.
• W2104813426 cites W2017701005 @default.
• W2104813426 cites W2030695915 @default.
• W2104813426 cites W2031226358 @default.
• W2104813426 cites W2047174634 @default.
• W2104813426 cites W2054738365 @default.
• W2104813426 cites W2057416949 @default.
• W2104813426 cites W2057911939 @default.
• W2104813426 cites W2064905503 @default.
• W2104813426 cites W2085771327 @default.
• W2104813426 cites W2088986886 @default.
• W2104813426 cites W2089939413 @default.
• W2104813426 cites W2111640904 @default.
• W2104813426 cites W2116115586 @default.
• W2104813426 cites W2125609096 @default.
• W2104813426 cites W2132990040 @default.
• W2104813426 cites W2133208043 @default.
• W2104813426 cites W2138306936 @default.
• W2104813426 cites W2154466172 @default.
• W2104813426 cites W2159133182 @default.
• W2104813426 cites W2171463471 @default.
• W2104813426 cites W2171529481 @default.
• W2104813426 cites W2171996869 @default.
• W2104813426 cites W4233808990 @default.
• W2104813426 cites W4244151339 @default.
• W2104813426 doi "https://doi.org/10.1002/elsc.201000161" @default.
• W2104813426 hasPublicationYear "2011" @default.
• W2104813426 type Work @default.
• W2104813426 sameAs 2104813426 @default.
• W2104813426 citedByCount "9" @default.
• W2104813426 countsByYear W21048134262012 @default.
• W2104813426 countsByYear W21048134262013 @default.
• W2104813426 countsByYear W21048134262014 @default.
• W2104813426 countsByYear W21048134262015 @default.
• W2104813426 countsByYear W21048134262017 @default.
• W2104813426 countsByYear W21048134262021 @default.
• W2104813426 crossrefType "journal-article" @default.
• W2104813426 hasAuthorship W2104813426A5005359953 @default.
• W2104813426 hasAuthorship W2104813426A5031893561 @default.
• W2104813426 hasAuthorship W2104813426A5072037290 @default.
• W2104813426 hasAuthorship W2104813426A5078624374 @default.
• W2104813426 hasConcept C104317684 @default.
• W2104813426 hasConcept C113196181 @default.
• W2104813426 hasConcept C115540264 @default.
• W2104813426 hasConcept C142613039 @default.
• W2104813426 hasConcept C168170006 @default.
• W2104813426 hasConcept C178790620 @default.
• W2104813426 hasConcept C185592680 @default.
• W2104813426 hasConcept C18903297 @default.
• W2104813426 hasConcept C2776970464 @default.
• W2104813426 hasConcept C2777289219 @default.
• W2104813426 hasConcept C2780989563 @default.
• W2104813426 hasConcept C43617362 @default.
• W2104813426 hasConcept C523546767 @default.
• W2104813426 hasConcept C54355233 @default.
• W2104813426 hasConcept C55493867 @default.
• W2104813426 hasConcept C6557445 @default.
• W2104813426 hasConcept C86803240 @default.
• W2104813426 hasConceptScore W2104813426C104317684 @default.
• W2104813426 hasConceptScore W2104813426C113196181 @default.
• W2104813426 hasConceptScore W2104813426C115540264 @default.
• W2104813426 hasConceptScore W2104813426C142613039 @default.
• W2104813426 hasConceptScore W2104813426C168170006 @default.
• W2104813426 hasConceptScore W2104813426C178790620 @default.
• W2104813426 hasConceptScore W2104813426C185592680 @default.
• W2104813426 hasConceptScore W2104813426C18903297 @default.
• W2104813426 hasConceptScore W2104813426C2776970464 @default.
• W2104813426 hasConceptScore W2104813426C2777289219 @default.
• W2104813426 hasConceptScore W2104813426C2780989563 @default.
• W2104813426 hasConceptScore W2104813426C43617362 @default.
• W2104813426 hasConceptScore W2104813426C523546767 @default.
• W2104813426 hasConceptScore W2104813426C54355233 @default.
• W2104813426 hasConceptScore W2104813426C55493867 @default.
• W2104813426 hasConceptScore W2104813426C6557445 @default.
• W2104813426 hasConceptScore W2104813426C86803240 @default.
• W2104813426 hasLocation W21048134261 @default.
• W2104813426 hasOpenAccess W2104813426 @default.
• W2104813426 hasPrimaryLocation W21048134261 @default.
• W2104813426 hasRelatedWork W1520675189 @default.
• W2104813426 hasRelatedWork W1969128187 @default.
• W2104813426 hasRelatedWork W1973674229 @default.
• W2104813426 hasRelatedWork W1987635712 @default.
• W2104813426 hasRelatedWork W2038173429 @default.
• W2104813426 hasRelatedWork W2040088312 @default.

• next