FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2104894182> ?p ?o ?g. }

• W2104894182 endingPage "1497" @default.
• W2104894182 startingPage "1491" @default.
• W2104894182 abstract "ABSTRACT The transmembrane regulatory protein ToxR is required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae , including cholera toxin (CT) and the toxin coregulated pilus (TCP). ToxR is necessary for transcription of the gene encoding a second regulatory protein, ToxT, which is the direct transcriptional activator of CT and TCP genes. However, ToxR, independent of ToxT, directly activates and represses transcription of the outer membrane porins OmpU and OmpT, respectively. The genes encoding TCP and CT (and including ToxT) lie on horizontally acquired genetic elements, while the toxR , ompU , and ompT genes are apparently in the ancestral Vibrio chromosome. The contribution of ToxR-dependent modulation of outer membrane porins to cholera pathogenesis has remained unknown. We demonstrate that ToxR mediates enhanced bile resistance in a ToxT-independent manner. In both classical and El Tor biotypes of V. cholerae , a toxR mutant strain has a reduced minimum bactericidal concentration (MBC) of bile, the bile component deoxycholate (DC), and the anionic detergent sodium dodecyl sulfate (SDS) compared to both wild-type and toxT mutant strains. Classical and El Tor toxR mutant strains also exhibit reduced growth rates at subinhibitory concentrations of DC and SDS. Growth of either V. cholerae biotype in subinhibitory concentrations of bile or DC induces increased ToxR-dependent production of a major 38-kDa outer membrane protein, which was confirmed to be OmpU by Western blot. Measurement of transcription of a ompU p- lacZ fusion in both biotypes reveals stimulation (about two- to threefold) of ToxR-dependent ompU transcription by the presence of bile or DC, suggesting that ToxR may respond to the presence of bile. The toxR mutant strains of three additional human intestinal pathogenic Vibrio species, V. mimicus , V. fluvialis , and V. parahaemolyticus , display lower MBCs of bile, DC, and SDS and have altered outer membrane protein profiles compared to the parental wild-type strains. Our results demonstrate a conserved role for ToxR in the modulation of outer membrane proteins and bile resistance of pathogenic Vibrio species and suggest that these ToxR-dependent outer membrane proteins may mediate enhanced resistance to bile. We speculate that ToxR-mediated bile resistance was an early step in the evolution of V. cholerae as an intestinal pathogen." @default.
• W2104894182 created "2016-06-24" @default.
• W2104894182 creator A5033454157 @default.
• W2104894182 creator A5042034255 @default.
• W2104894182 creator A5052146584 @default.
• W2104894182 creator A5053219723 @default.
• W2104894182 date "2000-03-01" @default.
• W2104894182 modified "2023-09-27" @default.
• W2104894182 title "The Virulence Regulatory Protein ToxR Mediates Enhanced Bile Resistance in <i>Vibrio cholerae</i> and Other Pathogenic <i>Vibrio</i> Species" @default.
• W2104894182 cites W1539475140 @default.
• W2104894182 cites W1542817241 @default.
• W2104894182 cites W1654724096 @default.
• W2104894182 cites W1787690277 @default.
• W2104894182 cites W1930763882 @default.
• W2104894182 cites W1939312344 @default.
• W2104894182 cites W1977151336 @default.
• W2104894182 cites W1995445964 @default.
• W2104894182 cites W2001304636 @default.
• W2104894182 cites W2006628821 @default.
• W2104894182 cites W2019171401 @default.
• W2104894182 cites W2033289414 @default.
• W2104894182 cites W2070322161 @default.
• W2104894182 cites W2093201831 @default.
• W2104894182 cites W2095835814 @default.
• W2104894182 cites W2104196289 @default.
• W2104894182 cites W2105882272 @default.
• W2104894182 cites W2110914325 @default.
• W2104894182 cites W2112502782 @default.
• W2104894182 cites W2118248940 @default.
• W2104894182 cites W2119127986 @default.
• W2104894182 cites W2122735072 @default.
• W2104894182 cites W2130380516 @default.
• W2104894182 cites W2132417094 @default.
• W2104894182 cites W2134042260 @default.
• W2104894182 cites W2145502538 @default.
• W2104894182 cites W2149298707 @default.
• W2104894182 cites W2152817710 @default.
• W2104894182 cites W2159393451 @default.
• W2104894182 cites W2160084442 @default.
• W2104894182 cites W2160321020 @default.
• W2104894182 doi "https://doi.org/10.1128/iai.68.3.1491-1497.2000" @default.
• W2104894182 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/97306" @default.
• W2104894182 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10678965" @default.
• W2104894182 hasPublicationYear "2000" @default.
• W2104894182 type Work @default.
• W2104894182 sameAs 2104894182 @default.
• W2104894182 citedByCount "158" @default.
• W2104894182 countsByYear W21048941822012 @default.
• W2104894182 countsByYear W21048941822013 @default.
• W2104894182 countsByYear W21048941822014 @default.
• W2104894182 countsByYear W21048941822015 @default.
• W2104894182 countsByYear W21048941822016 @default.
• W2104894182 countsByYear W21048941822017 @default.
• W2104894182 countsByYear W21048941822018 @default.
• W2104894182 countsByYear W21048941822019 @default.
• W2104894182 countsByYear W21048941822020 @default.
• W2104894182 countsByYear W21048941822021 @default.
• W2104894182 countsByYear W21048941822022 @default.
• W2104894182 countsByYear W21048941822023 @default.
• W2104894182 crossrefType "journal-article" @default.
• W2104894182 hasAuthorship W2104894182A5033454157 @default.
• W2104894182 hasAuthorship W2104894182A5042034255 @default.
• W2104894182 hasAuthorship W2104894182A5052146584 @default.
• W2104894182 hasAuthorship W2104894182A5053219723 @default.
• W2104894182 hasBestOaLocation W21048941821 @default.
• W2104894182 hasConcept C104317684 @default.
• W2104894182 hasConcept C143065580 @default.
• W2104894182 hasConcept C146587185 @default.
• W2104894182 hasConcept C153911025 @default.
• W2104894182 hasConcept C174832134 @default.
• W2104894182 hasConcept C2776422119 @default.
• W2104894182 hasConcept C2777006276 @default.
• W2104894182 hasConcept C2778643871 @default.
• W2104894182 hasConcept C2781217973 @default.
• W2104894182 hasConcept C523546767 @default.
• W2104894182 hasConcept C54355233 @default.
• W2104894182 hasConcept C547475151 @default.
• W2104894182 hasConcept C60987743 @default.
• W2104894182 hasConcept C62130838 @default.
• W2104894182 hasConcept C86803240 @default.
• W2104894182 hasConcept C89423630 @default.
• W2104894182 hasConceptScore W2104894182C104317684 @default.
• W2104894182 hasConceptScore W2104894182C143065580 @default.
• W2104894182 hasConceptScore W2104894182C146587185 @default.
• W2104894182 hasConceptScore W2104894182C153911025 @default.
• W2104894182 hasConceptScore W2104894182C174832134 @default.
• W2104894182 hasConceptScore W2104894182C2776422119 @default.
• W2104894182 hasConceptScore W2104894182C2777006276 @default.
• W2104894182 hasConceptScore W2104894182C2778643871 @default.
• W2104894182 hasConceptScore W2104894182C2781217973 @default.
• W2104894182 hasConceptScore W2104894182C523546767 @default.
• W2104894182 hasConceptScore W2104894182C54355233 @default.
• W2104894182 hasConceptScore W2104894182C547475151 @default.
• W2104894182 hasConceptScore W2104894182C60987743 @default.
• W2104894182 hasConceptScore W2104894182C62130838 @default.
• W2104894182 hasConceptScore W2104894182C86803240 @default.
• W2104894182 hasConceptScore W2104894182C89423630 @default.
• W2104894182 hasIssue "3" @default.

• next