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• W2105019069 abstract "The on-line coupling of Raman spectroscopy and conventional-size liquid chromatography (LC) by using a plastic liquid core waveguide (LCW) was explored. The LCW considered (length 30 cm, internal diameter 280 μm, internal volume 18 μL) is suitable for light guiding through aqueous LC eluents since the refractive index of the plastic perfluorinated material (denoted as Teflon AF2400) is 1.29, i.e., smaller than that of water (1.333) or methanol (1.329). Three-dimensional chromatograms showing on-the-fly Raman spectra are shown for a test mixture of 4-nitroaniline, 4-nitrophenol, 2,4-dinitrophenol, and nitrobenzene, compounds with slightly different NO2 vibrations. A limit of detection of 10 μg/mL for 4-nitroaniline, the most favorable of the test compounds, was achieved; the linearity ranged from 0.05 to 3.0 mg/mL (six data points in duplicate, r2 = 0.997). The improved detectability is due to the increased optical path length provided by the LCW and to the use of a large-volume-injection procedure (500-μL injections which effected a 35-fold improvement compared to standard, 10 μL, injections). The results of this preliminary study indicate that LC−Raman has potential." @default.
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• W2105019069 date "1999-09-08" @default.
• W2105019069 modified "2023-10-05" @default.
• W2105019069 title "On-Line Coupling of Column Liquid Chromatography and Raman Spectroscopy Using a Liquid Core Waveguide" @default.
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• W2105019069 doi "https://doi.org/10.1021/ac9902648" @default.
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