Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105089062> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2105089062 endingPage "366" @default.
- W2105089062 startingPage "359" @default.
- W2105089062 abstract "We have adapted an Ortho ICP-22 flow cytometer (Ortho Instruments, Westwood MA) for the simultaneous measurement of three independent fluorochromes and cell volume. This has been accomplished by the addition of a third photomultiplier tube and the development of a new electronic cell volume (ECV) flow cell. Cells are first analyzed as they pass through the 100 U ECV aperture and are then excited approximately 15 μsec later by the 365 nm mercury arc beam reflected by a 400 nm dicroic mirror. Independent blue, green and red signals can be associated by a delay circuit to the ECV signal from the same cell. We have developed this system as an aid in the analysis of tumor cell and macrophage heterogeneity and differentiation. The choice of stain combinations to be used is extremely flexible and permits the anlysis of a wide range of enzyme activities in conjunction with DNA/RNA and phagocytic probes. Data presented indicates the value of this approach in identifying the presence of plasminogen activator-like activity in both tumor and inflammatory cells within a malignant effusion as well as the quantitative expression of a number of markers of macrophage differentiation. Although the described techniques have been developed on a mercury are instrument, they can be used equally well with cell sorters." @default.
- W2105089062 created "2016-06-24" @default.
- W2105089062 creator A5010406153 @default.
- W2105089062 creator A5014460655 @default.
- W2105089062 creator A5019529854 @default.
- W2105089062 creator A5059572479 @default.
- W2105089062 creator A5087566251 @default.
- W2105089062 creator A5089434284 @default.
- W2105089062 date "1983-03-01" @default.
- W2105089062 modified "2023-09-23" @default.
- W2105089062 title "Simultaneous three color and electronic cell volume analysis with a single uv excitation source" @default.
- W2105089062 cites W1968166929 @default.
- W2105089062 cites W1973548878 @default.
- W2105089062 cites W1974548614 @default.
- W2105089062 cites W1983927512 @default.
- W2105089062 cites W2066638979 @default.
- W2105089062 cites W2071463524 @default.
- W2105089062 cites W2087922325 @default.
- W2105089062 cites W2088059732 @default.
- W2105089062 cites W2088492857 @default.
- W2105089062 cites W2131204347 @default.
- W2105089062 cites W2146273792 @default.
- W2105089062 cites W2153847357 @default.
- W2105089062 cites W2164174189 @default.
- W2105089062 cites W4299130242 @default.
- W2105089062 doi "https://doi.org/10.1002/cyto.990030510" @default.
- W2105089062 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6188588" @default.
- W2105089062 hasPublicationYear "1983" @default.
- W2105089062 type Work @default.
- W2105089062 sameAs 2105089062 @default.
- W2105089062 citedByCount "11" @default.
- W2105089062 countsByYear W21050890622018 @default.
- W2105089062 crossrefType "journal-article" @default.
- W2105089062 hasAuthorship W2105089062A5010406153 @default.
- W2105089062 hasAuthorship W2105089062A5014460655 @default.
- W2105089062 hasAuthorship W2105089062A5019529854 @default.
- W2105089062 hasAuthorship W2105089062A5059572479 @default.
- W2105089062 hasAuthorship W2105089062A5087566251 @default.
- W2105089062 hasAuthorship W2105089062A5089434284 @default.
- W2105089062 hasBestOaLocation W21050890621 @default.
- W2105089062 hasConcept C113879476 @default.
- W2105089062 hasConcept C120665830 @default.
- W2105089062 hasConcept C121332964 @default.
- W2105089062 hasConcept C136229726 @default.
- W2105089062 hasConcept C1491633281 @default.
- W2105089062 hasConcept C153911025 @default.
- W2105089062 hasConcept C185592680 @default.
- W2105089062 hasConcept C553184892 @default.
- W2105089062 hasConcept C55493867 @default.
- W2105089062 hasConcept C71924100 @default.
- W2105089062 hasConcept C86803240 @default.
- W2105089062 hasConcept C94915269 @default.
- W2105089062 hasConceptScore W2105089062C113879476 @default.
- W2105089062 hasConceptScore W2105089062C120665830 @default.
- W2105089062 hasConceptScore W2105089062C121332964 @default.
- W2105089062 hasConceptScore W2105089062C136229726 @default.
- W2105089062 hasConceptScore W2105089062C1491633281 @default.
- W2105089062 hasConceptScore W2105089062C153911025 @default.
- W2105089062 hasConceptScore W2105089062C185592680 @default.
- W2105089062 hasConceptScore W2105089062C553184892 @default.
- W2105089062 hasConceptScore W2105089062C55493867 @default.
- W2105089062 hasConceptScore W2105089062C71924100 @default.
- W2105089062 hasConceptScore W2105089062C86803240 @default.
- W2105089062 hasConceptScore W2105089062C94915269 @default.
- W2105089062 hasIssue "5" @default.
- W2105089062 hasLocation W21050890621 @default.
- W2105089062 hasLocation W21050890622 @default.
- W2105089062 hasOpenAccess W2105089062 @default.
- W2105089062 hasPrimaryLocation W21050890621 @default.
- W2105089062 hasRelatedWork W1686076540 @default.
- W2105089062 hasRelatedWork W1903055505 @default.
- W2105089062 hasRelatedWork W1971266684 @default.
- W2105089062 hasRelatedWork W2389193565 @default.
- W2105089062 hasRelatedWork W2399835922 @default.
- W2105089062 hasRelatedWork W2417824747 @default.
- W2105089062 hasRelatedWork W2469079696 @default.
- W2105089062 hasRelatedWork W4232334681 @default.
- W2105089062 hasRelatedWork W4291127362 @default.
- W2105089062 hasRelatedWork W4313373592 @default.
- W2105089062 hasVolume "3" @default.
- W2105089062 isParatext "false" @default.
- W2105089062 isRetracted "false" @default.
- W2105089062 magId "2105089062" @default.
- W2105089062 workType "article" @default.