Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105192600> ?p ?o ?g. }
- W2105192600 endingPage "223" @default.
- W2105192600 startingPage "215" @default.
- W2105192600 abstract "Purpose: To assess the in-vitro effects of dexamethasone (DEX) on the proliferation, apoptosis, and Na+-K+-ATPase activity of bovine corneal endothelial cells. Methods: Bovine corneal endothelial cells were cultured with DEX ranging from 10−10 to 10−3 M. The effect of DEX on the proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay. Apoptosis and necrosis were detected by staining with fluorescein-conjugated annexin V and propidium iodide, followed by flow cytometry. The effect of DEX on Na+-K+-ATPase activity was evaluated using non-isotopic methods. Results: DEX did not affect cellular proliferation or induce apoptosis/necrosis from 10−10 to 10−5 M. At 10−4 and 10−3 M, DEX significantly decreased proliferation and increased apoptosis and/or necrosis. DEX significantly increased the Na+-K+-ATPase activity from 10−8 to 10−6 M, with the maximal effect at 10−6 M (p < 0.01); this effect was inhibited by RU38486, an antiglucocorticoid molecule. Conclusions: Bovine corneal endothelial cells express glucocorticoid receptor (GR) mRNA and protein. DEX decreases cell proliferation and induces cellular apoptosis and/or necrosis at high concentrations. DEX also increases the Na+-K+-ATPase activity at certain concentrations." @default.
- W2105192600 created "2016-06-24" @default.
- W2105192600 creator A5022991559 @default.
- W2105192600 creator A5025063595 @default.
- W2105192600 creator A5030875110 @default.
- W2105192600 creator A5053636127 @default.
- W2105192600 creator A5053657893 @default.
- W2105192600 creator A5070346853 @default.
- W2105192600 creator A5081346472 @default.
- W2105192600 date "2006-01-01" @default.
- W2105192600 modified "2023-10-16" @default.
- W2105192600 title "In-Vitro Effects of Dexamethasone on Cellular Proliferation, Apoptosis, and Na+-K+-ATPase Activity of Bovine Corneal Endothelial Cells" @default.
- W2105192600 cites W1499029873 @default.
- W2105192600 cites W1556961396 @default.
- W2105192600 cites W1559184092 @default.
- W2105192600 cites W1579085732 @default.
- W2105192600 cites W1824926988 @default.
- W2105192600 cites W1964581876 @default.
- W2105192600 cites W1969273792 @default.
- W2105192600 cites W1974517355 @default.
- W2105192600 cites W1983344470 @default.
- W2105192600 cites W1983748037 @default.
- W2105192600 cites W1991588414 @default.
- W2105192600 cites W1998953959 @default.
- W2105192600 cites W2004008679 @default.
- W2105192600 cites W2008457039 @default.
- W2105192600 cites W2010277922 @default.
- W2105192600 cites W2012868183 @default.
- W2105192600 cites W2013955069 @default.
- W2105192600 cites W2015339982 @default.
- W2105192600 cites W2020528440 @default.
- W2105192600 cites W2025295035 @default.
- W2105192600 cites W2033709866 @default.
- W2105192600 cites W2041512194 @default.
- W2105192600 cites W2043736834 @default.
- W2105192600 cites W2050275562 @default.
- W2105192600 cites W2052290897 @default.
- W2105192600 cites W2052500168 @default.
- W2105192600 cites W2054306576 @default.
- W2105192600 cites W2059426569 @default.
- W2105192600 cites W2060903877 @default.
- W2105192600 cites W2064372503 @default.
- W2105192600 cites W2066083595 @default.
- W2105192600 cites W2072517813 @default.
- W2105192600 cites W2074675001 @default.
- W2105192600 cites W2081684816 @default.
- W2105192600 cites W2085582905 @default.
- W2105192600 cites W2095728767 @default.
- W2105192600 cites W2101082219 @default.
- W2105192600 cites W2107081701 @default.
- W2105192600 cites W2117658374 @default.
- W2105192600 cites W2167223040 @default.
- W2105192600 cites W2221309829 @default.
- W2105192600 cites W2225263353 @default.
- W2105192600 cites W2323709605 @default.
- W2105192600 cites W2415358415 @default.
- W2105192600 cites W4250414699 @default.
- W2105192600 cites W4254506960 @default.
- W2105192600 cites W4300548237 @default.
- W2105192600 doi "https://doi.org/10.1080/09273940600732380" @default.
- W2105192600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16911983" @default.
- W2105192600 hasPublicationYear "2006" @default.
- W2105192600 type Work @default.
- W2105192600 sameAs 2105192600 @default.
- W2105192600 citedByCount "45" @default.
- W2105192600 countsByYear W21051926002012 @default.
- W2105192600 countsByYear W21051926002013 @default.
- W2105192600 countsByYear W21051926002014 @default.
- W2105192600 countsByYear W21051926002015 @default.
- W2105192600 countsByYear W21051926002016 @default.
- W2105192600 countsByYear W21051926002017 @default.
- W2105192600 countsByYear W21051926002018 @default.
- W2105192600 countsByYear W21051926002019 @default.
- W2105192600 countsByYear W21051926002020 @default.
- W2105192600 countsByYear W21051926002021 @default.
- W2105192600 countsByYear W21051926002023 @default.
- W2105192600 crossrefType "journal-article" @default.
- W2105192600 hasAuthorship W2105192600A5022991559 @default.
- W2105192600 hasAuthorship W2105192600A5025063595 @default.
- W2105192600 hasAuthorship W2105192600A5030875110 @default.
- W2105192600 hasAuthorship W2105192600A5053636127 @default.
- W2105192600 hasAuthorship W2105192600A5053657893 @default.
- W2105192600 hasAuthorship W2105192600A5070346853 @default.
- W2105192600 hasAuthorship W2105192600A5081346472 @default.
- W2105192600 hasConcept C126322002 @default.
- W2105192600 hasConcept C134018914 @default.
- W2105192600 hasConcept C153911025 @default.
- W2105192600 hasConcept C185592680 @default.
- W2105192600 hasConcept C190283241 @default.
- W2105192600 hasConcept C2775934118 @default.
- W2105192600 hasConcept C2780004078 @default.
- W2105192600 hasConcept C2780401358 @default.
- W2105192600 hasConcept C2780841215 @default.
- W2105192600 hasConcept C31573885 @default.
- W2105192600 hasConcept C55493867 @default.
- W2105192600 hasConcept C59493245 @default.
- W2105192600 hasConcept C62112901 @default.
- W2105192600 hasConcept C71924100 @default.