Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105193670> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2105193670 endingPage "40" @default.
- W2105193670 startingPage "31" @default.
- W2105193670 abstract "An iso-osmotic Percoll density gradient was applied to determine the subcellular localization of the O2- generating enzyme, NADPH oxidase, in guinea pig polymorphonuclear leukocytes (PMN). [14C]Myristate (MA) was employed as a metabolic stimulant in order to clarify whether the myristate binding site on PMN membrane was identical with the O2- generating site. The distribution pattern of the O2- generating activity of MA-activated PMN was compared with that of unactivated PMN in parallel experiments to find fractions showing an enhanced O2- generating activity (i.e., above the background values due to O2-generation by other electron-transport systems). We observed very high O2- generating activity concentrated in a single peak with MA-activated PMN but little activity was seen with unactivated PMN in the Percoll density gradient. The O2- generating activity of MA-activated PMN was consistently associated with 5'-nucleotidase activity as a membrane marker enzyme, but was not associated with lysosomal marker enzymes such as myeloper-oxidase and lysozyme. O2- generating and 5'-nucleotidase activities in the peak fraction of MA-activated PMN were increased to about six and four times those of whole cells in terms of specific activity, respectively. These results indicate that the O2- generating enzyme is located on PMN plasma membrane. Furthermore, [14C]myristate-binding activity was mainly found in the peak fraction containing O2 - generating enzyme. This suggests that [14C]myristate binds to plasma membrane, and the O2 - generating enzyme may thus be activated." @default.
- W2105193670 created "2016-06-24" @default.
- W2105193670 creator A5003787011 @default.
- W2105193670 creator A5018825058 @default.
- W2105193670 creator A5041205717 @default.
- W2105193670 creator A5075215511 @default.
- W2105193670 date "1982-01-01" @default.
- W2105193670 modified "2023-09-26" @default.
- W2105193670 title "Subcellular Localization of O2− Generating Enzyme in Guinea Pig Polymorphonuclear Leukocytes; Fractionation of Subcellular Particles by Using a Percoll Density Gradient" @default.
- W2105193670 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a133689" @default.
- W2105193670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6279584" @default.
- W2105193670 hasPublicationYear "1982" @default.
- W2105193670 type Work @default.
- W2105193670 sameAs 2105193670 @default.
- W2105193670 citedByCount "23" @default.
- W2105193670 crossrefType "journal-article" @default.
- W2105193670 hasAuthorship W2105193670A5003787011 @default.
- W2105193670 hasAuthorship W2105193670A5018825058 @default.
- W2105193670 hasAuthorship W2105193670A5041205717 @default.
- W2105193670 hasAuthorship W2105193670A5075215511 @default.
- W2105193670 hasConcept C181199279 @default.
- W2105193670 hasConcept C185592680 @default.
- W2105193670 hasConcept C203014093 @default.
- W2105193670 hasConcept C27239569 @default.
- W2105193670 hasConcept C2776966407 @default.
- W2105193670 hasConcept C2777913276 @default.
- W2105193670 hasConcept C2777983669 @default.
- W2105193670 hasConcept C2779719074 @default.
- W2105193670 hasConcept C28212737 @default.
- W2105193670 hasConcept C41625074 @default.
- W2105193670 hasConcept C48679268 @default.
- W2105193670 hasConcept C55493867 @default.
- W2105193670 hasConcept C7623868 @default.
- W2105193670 hasConcept C86803240 @default.
- W2105193670 hasConceptScore W2105193670C181199279 @default.
- W2105193670 hasConceptScore W2105193670C185592680 @default.
- W2105193670 hasConceptScore W2105193670C203014093 @default.
- W2105193670 hasConceptScore W2105193670C27239569 @default.
- W2105193670 hasConceptScore W2105193670C2776966407 @default.
- W2105193670 hasConceptScore W2105193670C2777913276 @default.
- W2105193670 hasConceptScore W2105193670C2777983669 @default.
- W2105193670 hasConceptScore W2105193670C2779719074 @default.
- W2105193670 hasConceptScore W2105193670C28212737 @default.
- W2105193670 hasConceptScore W2105193670C41625074 @default.
- W2105193670 hasConceptScore W2105193670C48679268 @default.
- W2105193670 hasConceptScore W2105193670C55493867 @default.
- W2105193670 hasConceptScore W2105193670C7623868 @default.
- W2105193670 hasConceptScore W2105193670C86803240 @default.
- W2105193670 hasIssue "1" @default.
- W2105193670 hasLocation W21051936701 @default.
- W2105193670 hasLocation W21051936702 @default.
- W2105193670 hasOpenAccess W2105193670 @default.
- W2105193670 hasPrimaryLocation W21051936701 @default.
- W2105193670 hasRelatedWork W1988259208 @default.
- W2105193670 hasRelatedWork W2022271875 @default.
- W2105193670 hasRelatedWork W2069650373 @default.
- W2105193670 hasRelatedWork W2081292821 @default.
- W2105193670 hasRelatedWork W2082849203 @default.
- W2105193670 hasRelatedWork W2105193670 @default.
- W2105193670 hasRelatedWork W2362589501 @default.
- W2105193670 hasRelatedWork W2372824606 @default.
- W2105193670 hasRelatedWork W2384792976 @default.
- W2105193670 hasRelatedWork W2767783620 @default.
- W2105193670 hasVolume "91" @default.
- W2105193670 isParatext "false" @default.
- W2105193670 isRetracted "false" @default.
- W2105193670 magId "2105193670" @default.
- W2105193670 workType "article" @default.