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• W2105263174 abstract "Several surgical approaches are being used to induce myocardial ischemia in rats. The present study investigated two different operative procedures in spontaneously breathing and mechanically ventilated rats under sham conditions. A snare around the left coronary artery (LCA) was achieved without occlusion. Left lateral thoracotomy was performed in spontaneously breathing and mechanically ventilated rats (tidal volume 8 ml/kg) with a respiratory rate of 90 strokes/min at different levels of O 2 supplementation (room air and 30, 40, and 90% O 2 ). All animals were observed for 60 min after thoracotomy. Rats operated with exteriorization of the heart through left lateral thoracotomy while breathing spontaneously developed severe hypoxia and hypercapnia despite an intrathoracic operation time of <1 min. Arterial O 2 content decreased from 18.7 ± 0.5 to 3.3 ± 0.9 vol%. Lactate increased from 1.2 ± 0.1 to 5.2 ± 0.3 mmol/l. Significant signs of ischemia were seen in the electrocardiogram up to 60 min. Mechanically ventilated animals exhibited a spectrum ranging from hypoxia (room air) to hyperoxia (90% O 2 ). In order not to jeopardize findings in experimental myocardial ischemia-reperfusion injury models, stable physiological parameters can be achieved in mechanically ventilated rats at an O 2 application of 30–40% at 90 strokes/min." @default.
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• W2105263174 date "1999-02-01" @default.
• W2105263174 modified "2023-10-01" @default.
• W2105263174 title "Surgical procedure affects physiological parameters in rat myocardial ischemia: need for mechanical ventilation" @default.
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• W2105263174 doi "https://doi.org/10.1152/ajpheart.1999.276.2.h472" @default.
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