FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105371121> ?p ?o ?g. }

• W2105371121 endingPage "583" @default.
• W2105371121 startingPage "575" @default.
• W2105371121 abstract "Oligosaccharyltransferase (OST) catalyzes the transfer of an oligosaccharide to an asparagine residue in polypeptide chains. Using positional scanning peptide libraries, we assessed the effects of amino acid variations on the in vitro glycosylation efficiency within and adjacent to an N-glycosylation consensus, Asn-X-Ser/Thr, with an archaeal OST from Pyrococcus furiosus. The amino acid variations at the X(-2), X(-1) and X(+1) positions in the sequence X(-2)-X(-1)-Asn-X-Ser/Thr-X(+1) strongly influenced the glycosylation efficiency to a similar extent at position X. The rank orders of the amino acid preferences were unique at each site. We experimentally confirmed that the archaeal OST does not require an acidic residue at the -2 position, unlike the eubacterial OSTs. Pro was disfavored at the -1 and +1 positions, although the exclusion was not as strict as that at X, whereas Pro was the most favored amino acid residue among those studied at the -2 position. The overall amino acid preferences are correlated with a conformational propensity to extend around the sequon. The results of the library experiments revealed that the optimal acceptor sequence was PYNVTK, with a K(m) of 10 µM. The heat-stable, single-subunit OST of P. furiosus is a potential candidate enzyme for the production of recombinant glycoproteins in bacterial cells. Quantitative assessment of the amino acid preferences of the OST enzyme will facilitate the proper design of a production system." @default.
• W2105371121 created "2016-06-24" @default.
• W2105371121 creator A5009313798 @default.
• W2105371121 creator A5083171167 @default.
• W2105371121 date "2010-11-29" @default.
• W2105371121 modified "2023-10-10" @default.
• W2105371121 title "Quantitative assessment of the preferences for the amino acid residues flanking archaeal N-linked glycosylation sites" @default.
• W2105371121 cites W1885211128 @default.
• W2105371121 cites W1947454816 @default.
• W2105371121 cites W1976104570 @default.
• W2105371121 cites W1987804131 @default.
• W2105371121 cites W1990133186 @default.
• W2105371121 cites W1996401266 @default.
• W2105371121 cites W2001929483 @default.
• W2105371121 cites W2004125187 @default.
• W2105371121 cites W2006631610 @default.
• W2105371121 cites W2007176408 @default.
• W2105371121 cites W2021533673 @default.
• W2105371121 cites W2043338013 @default.
• W2105371121 cites W2045506937 @default.
• W2105371121 cites W2045868744 @default.
• W2105371121 cites W2047048276 @default.
• W2105371121 cites W2048034139 @default.
• W2105371121 cites W2049122170 @default.
• W2105371121 cites W2063015454 @default.
• W2105371121 cites W2072287929 @default.
• W2105371121 cites W2073808757 @default.
• W2105371121 cites W2077417665 @default.
• W2105371121 cites W2077847564 @default.
• W2105371121 cites W2078703243 @default.
• W2105371121 cites W2081947865 @default.
• W2105371121 cites W2082693434 @default.
• W2105371121 cites W2088221752 @default.
• W2105371121 cites W2094848126 @default.
• W2105371121 cites W2107017911 @default.
• W2105371121 cites W2111354594 @default.
• W2105371121 cites W2129947778 @default.
• W2105371121 cites W2137297099 @default.
• W2105371121 cites W2145832204 @default.
• W2105371121 cites W2154378640 @default.
• W2105371121 cites W2159347606 @default.
• W2105371121 cites W2161171034 @default.
• W2105371121 cites W2163741463 @default.
• W2105371121 cites W2402846599 @default.
• W2105371121 doi "https://doi.org/10.1093/glycob/cwq196" @default.
• W2105371121 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21115605" @default.
• W2105371121 hasPublicationYear "2010" @default.
• W2105371121 type Work @default.
• W2105371121 sameAs 2105371121 @default.
• W2105371121 citedByCount "22" @default.
• W2105371121 countsByYear W21053711212012 @default.
• W2105371121 countsByYear W21053711212013 @default.
• W2105371121 countsByYear W21053711212014 @default.
• W2105371121 countsByYear W21053711212015 @default.
• W2105371121 countsByYear W21053711212017 @default.
• W2105371121 countsByYear W21053711212018 @default.
• W2105371121 countsByYear W21053711212019 @default.
• W2105371121 countsByYear W21053711212020 @default.
• W2105371121 countsByYear W21053711212021 @default.
• W2105371121 countsByYear W21053711212023 @default.
• W2105371121 crossrefType "journal-article" @default.
• W2105371121 hasAuthorship W2105371121A5009313798 @default.
• W2105371121 hasAuthorship W2105371121A5083171167 @default.
• W2105371121 hasBestOaLocation W21053711211 @default.
• W2105371121 hasConcept C104317684 @default.
• W2105371121 hasConcept C108625454 @default.
• W2105371121 hasConcept C167625842 @default.
• W2105371121 hasConcept C181199279 @default.
• W2105371121 hasConcept C185592680 @default.
• W2105371121 hasConcept C2775895851 @default.
• W2105371121 hasConcept C2776943354 @default.
• W2105371121 hasConcept C2777313579 @default.
• W2105371121 hasConcept C2781338088 @default.
• W2105371121 hasConcept C2994376932 @default.
• W2105371121 hasConcept C40767141 @default.
• W2105371121 hasConcept C515207424 @default.
• W2105371121 hasConcept C550995028 @default.
• W2105371121 hasConcept C55493867 @default.
• W2105371121 hasConcept C71240020 @default.
• W2105371121 hasConceptScore W2105371121C104317684 @default.
• W2105371121 hasConceptScore W2105371121C108625454 @default.
• W2105371121 hasConceptScore W2105371121C167625842 @default.
• W2105371121 hasConceptScore W2105371121C181199279 @default.
• W2105371121 hasConceptScore W2105371121C185592680 @default.
• W2105371121 hasConceptScore W2105371121C2775895851 @default.
• W2105371121 hasConceptScore W2105371121C2776943354 @default.
• W2105371121 hasConceptScore W2105371121C2777313579 @default.
• W2105371121 hasConceptScore W2105371121C2781338088 @default.
• W2105371121 hasConceptScore W2105371121C2994376932 @default.
• W2105371121 hasConceptScore W2105371121C40767141 @default.
• W2105371121 hasConceptScore W2105371121C515207424 @default.
• W2105371121 hasConceptScore W2105371121C550995028 @default.
• W2105371121 hasConceptScore W2105371121C55493867 @default.
• W2105371121 hasConceptScore W2105371121C71240020 @default.
• W2105371121 hasIssue "5" @default.
• W2105371121 hasLocation W21053711211 @default.
• W2105371121 hasLocation W21053711212 @default.
• W2105371121 hasOpenAccess W2105371121 @default.

• next