FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105412757> ?p ?o ?g. }

• W2105412757 abstract "Abstract Background It is now recognized that the neuro-vascular unit (NVU) plays a key role in several neurological diseases including epilepsy, stroke, Alzheimer’s disease, multiple sclerosis and the development of gliomas. Most of these disorders are associated with NVU dysfunction, due to overexpression of inflammatory factors such as vascular endothelial growth factor (VEGF). Various in vitro models have been developed previously to study the micro-environment of the blood–brain barrier (BBB). However none of these in vitro models contained a complete complement of NVU cells, nor maintained their interactions, thus minimizing the influence of the surrounding tissue on the BBB development and function. The organotypic hippocampal culture (OHC) is an integrative in vitro model that allows repeated manipulations over time to further understand the development of cell circuits or the mechanisms of brain diseases. Methods/design OHCs were cultured from hippocampi of 6–7 day-old Sprague Dawley rats. After 2 weeks in culture, seizures were induced by application of kainate or bicuculline into culture medium. The regulation of BBB integrity under physiological and pathological conditions was evaluated by immunostaining of the main tight junction (TJ) proteins and of the basal membrane of microvessels. To mimic or prevent BBB disassembly, we used diverse pro- or anti-angiogenic treatments. Discussion This study demonstrates that NVU regulation can be investigated using OHCs. We observed in this model system an increase in vascularization and a down-regulation of TJ proteins, similar to the vascular changes described in a chronic focus of epileptic patients, and in rodent models of epilepsy or inflammation. We observed that Zonula occludens-1 (ZO-1) protein disappeared after seizures associated with neuronal damage. In these conditions, the angiopoeitin-1 system was down-regulated, and the application of r-angiopoeitin-1 allowed TJ re-assembly. This article demonstrates that organotypic culture is a useful model to decipher the links between epileptic activity and vascular damage, and also to investigate NVU regulation in diverse neurological disorders." @default.
• W2105412757 created "2016-06-24" @default.
• W2105412757 creator A5004637139 @default.
• W2105412757 creator A5057049609 @default.
• W2105412757 creator A5075314368 @default.
• W2105412757 date "2013-02-07" @default.
• W2105412757 modified "2023-10-16" @default.
• W2105412757 title "Organotypic brain slices: a model to study the neurovascular unit micro-environment in epilepsies" @default.
• W2105412757 cites W120013794 @default.
• W2105412757 cites W1530235393 @default.
• W2105412757 cites W1539161723 @default.
• W2105412757 cites W1652900453 @default.
• W2105412757 cites W188961659 @default.
• W2105412757 cites W1967081185 @default.
• W2105412757 cites W1972794546 @default.
• W2105412757 cites W1980702346 @default.
• W2105412757 cites W1981311698 @default.
• W2105412757 cites W1986571652 @default.
• W2105412757 cites W1989900532 @default.
• W2105412757 cites W1992112921 @default.
• W2105412757 cites W1992866322 @default.
• W2105412757 cites W1996199379 @default.
• W2105412757 cites W2001267675 @default.
• W2105412757 cites W2003726414 @default.
• W2105412757 cites W2004365491 @default.
• W2105412757 cites W2006035554 @default.
• W2105412757 cites W2006359632 @default.
• W2105412757 cites W2008499380 @default.
• W2105412757 cites W2008625318 @default.
• W2105412757 cites W2010239869 @default.
• W2105412757 cites W2015541518 @default.
• W2105412757 cites W2015986915 @default.
• W2105412757 cites W2020117730 @default.
• W2105412757 cites W2022120753 @default.
• W2105412757 cites W2022307232 @default.
• W2105412757 cites W2022729064 @default.
• W2105412757 cites W2025688778 @default.
• W2105412757 cites W2029857151 @default.
• W2105412757 cites W2031062821 @default.
• W2105412757 cites W2036811411 @default.
• W2105412757 cites W2039836896 @default.
• W2105412757 cites W2049623666 @default.
• W2105412757 cites W2050481094 @default.
• W2105412757 cites W2050516456 @default.
• W2105412757 cites W2053721734 @default.
• W2105412757 cites W2054868504 @default.
• W2105412757 cites W2054903733 @default.
• W2105412757 cites W2063038783 @default.
• W2105412757 cites W2065154189 @default.
• W2105412757 cites W2067179973 @default.
• W2105412757 cites W2067349066 @default.
• W2105412757 cites W2068337543 @default.
• W2105412757 cites W2068879074 @default.
• W2105412757 cites W2069800856 @default.
• W2105412757 cites W2070812255 @default.
• W2105412757 cites W2072227193 @default.
• W2105412757 cites W2073772517 @default.
• W2105412757 cites W2077673959 @default.
• W2105412757 cites W2078652548 @default.
• W2105412757 cites W2079054696 @default.
• W2105412757 cites W2081309287 @default.
• W2105412757 cites W2082153274 @default.
• W2105412757 cites W2086026895 @default.
• W2105412757 cites W2087102885 @default.
• W2105412757 cites W2088027239 @default.
• W2105412757 cites W2109644106 @default.
• W2105412757 cites W2110463517 @default.
• W2105412757 cites W2112937635 @default.
• W2105412757 cites W2117691576 @default.
• W2105412757 cites W2124743446 @default.
• W2105412757 cites W2126641956 @default.
• W2105412757 cites W2133662527 @default.
• W2105412757 cites W2141077451 @default.
• W2105412757 cites W2148443137 @default.
• W2105412757 cites W2159821105 @default.
• W2105412757 cites W2168019328 @default.
• W2105412757 cites W2168956427 @default.
• W2105412757 cites W2170724437 @default.
• W2105412757 cites W2292619439 @default.
• W2105412757 cites W2400167934 @default.
• W2105412757 cites W4249085558 @default.
• W2105412757 doi "https://doi.org/10.1186/2045-8118-10-11" @default.
• W2105412757 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3605299" @default.
• W2105412757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23391266" @default.
• W2105412757 hasPublicationYear "2013" @default.
• W2105412757 type Work @default.
• W2105412757 sameAs 2105412757 @default.
• W2105412757 citedByCount "26" @default.
• W2105412757 countsByYear W21054127572013 @default.
• W2105412757 countsByYear W21054127572014 @default.
• W2105412757 countsByYear W21054127572015 @default.
• W2105412757 countsByYear W21054127572016 @default.
• W2105412757 countsByYear W21054127572017 @default.
• W2105412757 countsByYear W21054127572018 @default.
• W2105412757 countsByYear W21054127572019 @default.
• W2105412757 countsByYear W21054127572020 @default.
• W2105412757 countsByYear W21054127572021 @default.
• W2105412757 countsByYear W21054127572022 @default.
• W2105412757 countsByYear W21054127572023 @default.
• W2105412757 crossrefType "journal-article" @default.

• next