Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105483764> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2105483764 endingPage "550" @default.
- W2105483764 startingPage "545" @default.
- W2105483764 abstract "A chimeric human IgE monoclonal antibody was developed against the house dust mite allergen Der p 2. This chimeric antibody (hIgE-Dp2A) was composed of the heavy-chain variable domains and light chains of the original murine monoclonal antibody retaining its binding characteristics, whereas the heavy-chain constant domains were exchanged with the human IgE heavy chain. The chimeric IgE expression level was IgE 600 IU/ml (1 IU = 2.4 ng/ml). The binding of the chimeric hIgE-Dp2A to mite extract was indistinguishable from that of the original mouse monoclonal antibody. Parallel dose-response curves were found when the binding of hIgE-Dp2A to mite extract and anti-IgE coupled to sepharose were compared. Binding levels were not identical; however, hIgE-Dp2A bound significantly better to the mite-extract sepharose. This result indicates that the commonly used anti-IgE on solid phase calibration systems may lead to an overestimation of the amount of allergen-specific IgE present in the serum sample. The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Dose-response curves of serial dilutions of patient samples were parallel with the hIgE-Dp2A dose-response curve, which indicates that hIgE-Dp2A behaves like natural IgE antibodies in binding to allergen coupled to solid phase. This antibody is well suited for use as a reference reagent in the RAST and enables the expression of the amount of allergen-specific IgE present in a patient sample in absolute amounts. (J Allergy Clin Immunol 1997;99:545-50.)" @default.
- W2105483764 created "2016-06-24" @default.
- W2105483764 creator A5006695127 @default.
- W2105483764 creator A5039190287 @default.
- W2105483764 creator A5063480905 @default.
- W2105483764 creator A5066498600 @default.
- W2105483764 creator A5069729938 @default.
- W2105483764 creator A5073842398 @default.
- W2105483764 date "1997-04-01" @default.
- W2105483764 modified "2023-10-06" @default.
- W2105483764 title "Production of a mouse/human chimeric IgE monoclonal antibody to the house dust mite allergen Der p 2 and its use for the absolute quantification of allergen-specific IgE" @default.
- W2105483764 cites W1599290645 @default.
- W2105483764 cites W168987915 @default.
- W2105483764 cites W1868085285 @default.
- W2105483764 cites W1968804518 @default.
- W2105483764 cites W1973580967 @default.
- W2105483764 cites W1986059203 @default.
- W2105483764 cites W2006572581 @default.
- W2105483764 cites W2014950129 @default.
- W2105483764 cites W2023095985 @default.
- W2105483764 cites W2032144081 @default.
- W2105483764 cites W2053595623 @default.
- W2105483764 cites W2061335193 @default.
- W2105483764 cites W2079218165 @default.
- W2105483764 cites W2106833014 @default.
- W2105483764 cites W2118929543 @default.
- W2105483764 cites W2144634347 @default.
- W2105483764 doi "https://doi.org/10.1016/s0091-6749(97)70083-6" @default.
- W2105483764 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9111501" @default.
- W2105483764 hasPublicationYear "1997" @default.
- W2105483764 type Work @default.
- W2105483764 sameAs 2105483764 @default.
- W2105483764 citedByCount "86" @default.
- W2105483764 countsByYear W21054837642013 @default.
- W2105483764 countsByYear W21054837642014 @default.
- W2105483764 countsByYear W21054837642016 @default.
- W2105483764 countsByYear W21054837642018 @default.
- W2105483764 countsByYear W21054837642021 @default.
- W2105483764 countsByYear W21054837642022 @default.
- W2105483764 countsByYear W21054837642023 @default.
- W2105483764 crossrefType "journal-article" @default.
- W2105483764 hasAuthorship W2105483764A5006695127 @default.
- W2105483764 hasAuthorship W2105483764A5039190287 @default.
- W2105483764 hasAuthorship W2105483764A5063480905 @default.
- W2105483764 hasAuthorship W2105483764A5066498600 @default.
- W2105483764 hasAuthorship W2105483764A5069729938 @default.
- W2105483764 hasAuthorship W2105483764A5073842398 @default.
- W2105483764 hasConcept C141105273 @default.
- W2105483764 hasConcept C153911025 @default.
- W2105483764 hasConcept C159654299 @default.
- W2105483764 hasConcept C185592680 @default.
- W2105483764 hasConcept C203014093 @default.
- W2105483764 hasConcept C207480886 @default.
- W2105483764 hasConcept C2778166374 @default.
- W2105483764 hasConcept C2780510475 @default.
- W2105483764 hasConcept C542903549 @default.
- W2105483764 hasConcept C71924100 @default.
- W2105483764 hasConcept C86803240 @default.
- W2105483764 hasConceptScore W2105483764C141105273 @default.
- W2105483764 hasConceptScore W2105483764C153911025 @default.
- W2105483764 hasConceptScore W2105483764C159654299 @default.
- W2105483764 hasConceptScore W2105483764C185592680 @default.
- W2105483764 hasConceptScore W2105483764C203014093 @default.
- W2105483764 hasConceptScore W2105483764C207480886 @default.
- W2105483764 hasConceptScore W2105483764C2778166374 @default.
- W2105483764 hasConceptScore W2105483764C2780510475 @default.
- W2105483764 hasConceptScore W2105483764C542903549 @default.
- W2105483764 hasConceptScore W2105483764C71924100 @default.
- W2105483764 hasConceptScore W2105483764C86803240 @default.
- W2105483764 hasIssue "4" @default.
- W2105483764 hasLocation W21054837641 @default.
- W2105483764 hasLocation W21054837642 @default.
- W2105483764 hasOpenAccess W2105483764 @default.
- W2105483764 hasPrimaryLocation W21054837641 @default.
- W2105483764 hasRelatedWork W1521955045 @default.
- W2105483764 hasRelatedWork W2037019085 @default.
- W2105483764 hasRelatedWork W2042692988 @default.
- W2105483764 hasRelatedWork W2054895034 @default.
- W2105483764 hasRelatedWork W2066867162 @default.
- W2105483764 hasRelatedWork W2083087446 @default.
- W2105483764 hasRelatedWork W2083739516 @default.
- W2105483764 hasRelatedWork W2112795145 @default.
- W2105483764 hasRelatedWork W2404949638 @default.
- W2105483764 hasRelatedWork W2973731954 @default.
- W2105483764 hasVolume "99" @default.
- W2105483764 isParatext "false" @default.
- W2105483764 isRetracted "false" @default.
- W2105483764 magId "2105483764" @default.
- W2105483764 workType "article" @default.