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• W2105528571 abstract "This thesis work aimed to investigate the assembly and budding of enveloped virus particles with focus on the fate of cellular proteins, present in or near the plasma membrane (PM) where the budding occurs. It was previously shown that compact viruses, like alphaviruses, with a covering outer protein coat, did not contain any cellular proteins in the envelope. However, cellular proteins were found in purified retroviral preparations and these proteins were thought to be specifically incorporated into the viral envelopes. We asked whether cellular proteins instead were randomly incorporated during viral budding at the PM and if selection of proteins occurred at all. Hence, we built up systems to metabolically label lipids and proteins in cells both before and during infection and production of virus particles, to isolate and purify PMs and released virus particles and to equalize the preparations to each other thus enabling direct comparable analyses of protein contents and amounts of proteins in the two samples. In this thesis we can, for the chosen model retroviruses Moloney murine leukaemia virus (Mo-MuLV) and Human immunodeficiency virus type-1 (HIV-1), show that the bulk of the cellular proteins, present in or near the budding sites, are in a non-selective manner incorporated into assembling and budding particles. Only a few proteins were excluded from or concentrated into the purified particles, as compared to isolated PMs, which was opposite to the general idea. Some viruses, including Human herpesvirus 6A (HHV-6A), have been associated with the autoimmune disease multiple sclerosis (MS). One theory for induction of the autoimmune reactions is that incorporated cellular proteins are presented to immune system cells together with viral peptides, leading to misdirected immune reactions. We asked whether HHV-6A incorporates cellular proteins during assembly and release of viral particles. Indeed, we could conclude that purified, intact and infectious HHV-6A particles contained host proteins, among them we identified clathrin, ezrin, actin, Tsg101 and the CD46 protein which is also used as a receptor for the virus. Altogether, our results suggest that the majority of the proteins, present at or near the assembly and budding sites of retroviral particles, are incorporated into the particles in a non-selective manner. The work also suggests that enveloped viruses belonging to other viral families incorporate cellular proteins and this opens up for questions regarding, for example, induction of autoimmune diseases. Till mina foraldrar Agnes och Elam LIST OF PUBLICATIONS This thesis is based on the following papers, which will be referred to in the text by their Roman numerals: I. Hammarstedt*, M., Wallengren*, K., Pedersen, K. W., Roos, N., and H. Garoff. 2000. Minimal exclusion of plasma membrane proteins during retroviral envelope formation. PNAS, 97:7527-7532. II. Hammarstedt, M. and H. Garoff. 2004. Passive and active inclusion of host proteins in Human immunodeficiency virus type 1 Gag particles during budding at the plasma membrane. J Virol, 78:5686-5697. III. Hammarstedt*, M., Ahlqvist*, J., Jacobson, S., Garoff, H. and A. FogdellHahn. Incorporation of cellular proteins into Human herpesvirus 6A. Submitted. The articles were reproduced with permission from the respective copyright holder. * Shared first authorship. TABLE OF CONTENTS" @default.
• W2105528571 created "2016-06-24" @default.
• W2105528571 creator A5081255013 @default.
• W2105528571 date "2006-11-10" @default.
• W2105528571 modified "2023-09-27" @default.
• W2105528571 title "Incorporation of cellular proteins into enveloped virus particles" @default.
• W2105528571 cites W1483155462 @default.
• W2105528571 cites W1491459594 @default.
• W2105528571 cites W1491787713 @default.
• W2105528571 cites W1505537700 @default.
• W2105528571 cites W1508924431 @default.
• W2105528571 cites W1517900121 @default.
• W2105528571 cites W1532225053 @default.
• W2105528571 cites W1542639585 @default.
• W2105528571 cites W1543183990 @default.
• W2105528571 cites W1544126343 @default.
• W2105528571 cites W1552228345 @default.
• W2105528571 cites W1579191879 @default.
• W2105528571 cites W1583571071 @default.
• W2105528571 cites W1592949353 @default.
• W2105528571 cites W1601232152 @default.
• W2105528571 cites W1608001605 @default.
• W2105528571 cites W1608128491 @default.
• W2105528571 cites W1611298747 @default.
• W2105528571 cites W1624246797 @default.
• W2105528571 cites W1632486555 @default.
• W2105528571 cites W1641229892 @default.
• W2105528571 cites W1643517094 @default.
• W2105528571 cites W1659840293 @default.
• W2105528571 cites W1664638011 @default.
• W2105528571 cites W170446279 @default.
• W2105528571 cites W1756426621 @default.
• W2105528571 cites W1760183277 @default.
• W2105528571 cites W1762245052 @default.
• W2105528571 cites W1776574444 @default.
• W2105528571 cites W1788853185 @default.
• W2105528571 cites W1806998329 @default.
• W2105528571 cites W1813266103 @default.
• W2105528571 cites W1844569659 @default.
• W2105528571 cites W1883977612 @default.
• W2105528571 cites W1889340880 @default.
• W2105528571 cites W1894956018 @default.
• W2105528571 cites W1905230553 @default.
• W2105528571 cites W1963768207 @default.
• W2105528571 cites W1964351701 @default.
• W2105528571 cites W1967406495 @default.
• W2105528571 cites W1968873679 @default.
• W2105528571 cites W1969077479 @default.
• W2105528571 cites W1970630169 @default.
• W2105528571 cites W1971907557 @default.
• W2105528571 cites W1972338658 @default.
• W2105528571 cites W1973642611 @default.
• W2105528571 cites W1973933701 @default.
• W2105528571 cites W1974224443 @default.
• W2105528571 cites W1974607456 @default.
• W2105528571 cites W1977282539 @default.
• W2105528571 cites W1977453669 @default.
• W2105528571 cites W1984587504 @default.
• W2105528571 cites W1985844089 @default.
• W2105528571 cites W1986015062 @default.
• W2105528571 cites W1989186015 @default.
• W2105528571 cites W1990035867 @default.
• W2105528571 cites W1991069890 @default.
• W2105528571 cites W1991655565 @default.
• W2105528571 cites W1991800507 @default.
• W2105528571 cites W1992031061 @default.
• W2105528571 cites W1993452562 @default.
• W2105528571 cites W1993877052 @default.
• W2105528571 cites W1994806672 @default.
• W2105528571 cites W1995634096 @default.
• W2105528571 cites W1996834128 @default.
• W2105528571 cites W2000058440 @default.
• W2105528571 cites W2001070158 @default.
• W2105528571 cites W2003793374 @default.
• W2105528571 cites W2008457983 @default.
• W2105528571 cites W2009465113 @default.
• W2105528571 cites W2009704023 @default.
• W2105528571 cites W2010279858 @default.
• W2105528571 cites W201050536 @default.
• W2105528571 cites W2012928044 @default.
• W2105528571 cites W2013241878 @default.
• W2105528571 cites W2018230968 @default.
• W2105528571 cites W2019987740 @default.
• W2105528571 cites W2020597127 @default.
• W2105528571 cites W2021015803 @default.
• W2105528571 cites W2021211941 @default.
• W2105528571 cites W2021501772 @default.
• W2105528571 cites W2023420720 @default.
• W2105528571 cites W2025042015 @default.
• W2105528571 cites W2026933314 @default.
• W2105528571 cites W2027962748 @default.
• W2105528571 cites W2028272667 @default.
• W2105528571 cites W2030634945 @default.
• W2105528571 cites W2032059035 @default.
• W2105528571 cites W2032181619 @default.
• W2105528571 cites W2033320393 @default.
• W2105528571 cites W2034030579 @default.
• W2105528571 cites W2034399323 @default.
• W2105528571 cites W2034577888 @default.
• W2105528571 cites W2034806350 @default.

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