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- W2105548610 abstract "Abstract: Leukocyte adhesion molecules play an important role in regulating immunocyte trafficking into peripheral tissues. CD 18 is the common β-chain of the β2-integrin class of leukocyte adhesion molecules. Porcine CD 18 cDNA was cloned using reverse transcriptase-polymerase chain reaction (RT-PCR), subcloned, and sequenced (GenBank accession number U13941). The deduced 769 amino acid sequence for porcine CD18 contains a 22 amino acid signal sequence, a cysteine-rich domain, and a 23 amino acid transmembrane domain. Porcine CD18 showed 79.3% cDNA sequence identity and 83.2% amino acid sequence identity with human CD 18. Northern blot analysis revealed that porcine CD 18 mRNA was expressed constitutively at high levels in freshly isolated alveolar macrophages, and levels declined approximately 50% over a 12 hr culture period. Peripheral blood cells and spleen cells expressed lower amounts of CD 18 mRNA. Anti-human and anti-canine CD 18 antibodies were tested for xeno-reactivity against porcine CD18, and more than 62% freshly isolated unstimulated alveolar macrophages were stained with anti-CD 18 antibodies. Porcine macrophage CD 18 transcript levels and cell surface expression detected by flow cytometry were not significantly altered following stimulation with LPS or human promflammatory cytokines, including interferon-γ(IFN-γ), interleukin-α (IL-1α), IL-1β, tumor necrosis factor-α (TNFα), and IL-6. Thus, CD 18 gene expression by porcine alveolar macrophages appears not to be regulated by human proinflammatory cytokines. These reagents may be useful for studying the role of adhesion molecules in the immunobiology of porcine models of allo- and xenotransplantation" @default.
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- W2105548610 date "1996-05-01" @default.
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- W2105548610 title "Molecular cloning and characterization of the porcine CD 18 leukocyte adhesion molecule" @default.
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- W2105548610 doi "https://doi.org/10.1111/j.1399-3089.1996.tb00141.x" @default.
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