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• W2105554562 abstract "To determine whether the rapid response of hepatic mRNA-Sl4 to carbohydrate feeding was associated with an alteration in a specific hepatic nucleotide, acid extracts of liver tissue were analyzed by ion-paired reverse phase HPLC. A nucleotide present in nanomoles per g quantity correlated with the induction of this mRNA by sucrose. Four hours after sucrose feeding the content of this compound was 7 times greater than that in fasted rats. Furthermore, the response was rapid, attaining maximal levels 30 min after feeding, and remaining elevated for at least 4 h. The content of this nucleotide also correlated with the content of mRNA-Sl4 in the steady state, with the lowest levels of both mRNA-Sl4 and this nucleotide in fasted rats, intermediate levels in rats maintained on a regular chow diet, and the highest levels in rats fed a 60% sucrose diet for 4 days. Lastly, while the response of mRNA-Sl4 to thyroid hormone and sucrose is synergistic, the thyroidal state does not influence the response of this compound to sucrose. Thus, the interaction of thyroid hormone and sucrose on mRNA-Sl4 is distal to the generation of this compound. Based on the UV spectrum, HPLC retention, and [3H]adenosine labeling, this compound contains adenine. However, it is not any of the common adenine-containing mono- or oligonucleotides. These data indicate that a novel hepatic nucleotide is induced by sucrose and raise the possibility that this nucleotide is responsible for the induction of carbohydrate-responsive mRNAs (Endocrinology124: 212–217, 1989)" @default.
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• W2105554562 date "1989-01-01" @default.
• W2105554562 modified "2023-09-25" @default.
• W2105554562 title "A Novel Hepatic Nucleotide Is Correlated with the Carbohydrate Induction of Messenger Ribonucleic Acid-S14*" @default.
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• W2105554562 doi "https://doi.org/10.1210/endo-124-1-212" @default.
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