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• W2105570852 abstract "We have previously described the construction of a P-selectin glycoprotein ligand-1—mouse immunoglobulin Fc fusion protein, which when transiently coexpressed with the porcine α1,3 galactosyltransferase in COS cells becomes a very efficient adsorber of xenoreactive, anti-pig antibodies. To relate the adsorption capacity with the glycan expression of individual fusion proteins produced in different cell lines, stable CHO-K1, COS, and 293T cells producing this fusion protein have been engineered. On α1,3 galactosyltransferase coexpression, high-affinity adsorbers were produced by both COS and 293T cells, whereas an adsorber of lower affinity was derived from CHO-K1 cells. Stable coexpression of a core 2 β1,6 N-acetylglucosaminyltransferase in CHO-K1 cells led to increased α-Gal epitope density and improved anti-pig antibody adsorption efficacy. ESI-MS/MS of O-glycans released from PSGL-1/mIgG2b produced in an α1,3 galactosyl- and core 2 β1,6 N-acetylglucosaminyltransferase expressing CHO-K1 cell clone revealed a number of structures with carbohydrate sequences consistent with terminal Gal-Gal. In contrast, no O-glycan structures with terminal Gal-Gal were identified on the fusion protein when expressed alone or in combination with the α1,3 galactosyltransferase in CHO-K1 cells. In conclusion, the density of α-Gal epitopes on PSGL-1/mIgG2b was dependent on the expression of O-linked glycans with core 2 structures and lactosamine extensions. The structural complexity of the terminal Gal-Gal expressing O-glycans with both neutral as well as sialic acid–containing structures is likely to contribute to the high adsorption efficacy." @default.
• W2105570852 created "2016-06-24" @default.
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• W2105570852 date "2004-12-29" @default.
• W2105570852 modified "2023-10-12" @default.
• W2105570852 title "Anti-pig antibody adsorption efficacy of α-Gal carrying recombinant P-selectin glycoprotein ligand-1/immunoglobulin chimeras increases with core 2 β1, 6-N-acetylglucosaminyltransferase expression" @default.
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• W2105570852 doi "https://doi.org/10.1093/glycob/cwi037" @default.
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