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- W2105588400 abstract "Francisella tularensis is a highly infectious human pathogen that can cause tularemia, but little is known about the lipid composition in membranes of this bacterium. Our recent study has shown that lipid A in membranes of F. tularensis subsp. novicida is related to its infection ability [X. Wang, A.A. Ribeiro, Z. Guan, S.N. Abraham, C.R. Raetz, Attenuated virulence of a Francisella mutant lacking the lipid A 4′-phosphatase, Proc. Natl. Acad. Sci. U.S.A. 104 (2007) 4136–4141.]. Here we purified the glycerophospholipids of F. novicida, and analyzed by using thin-layer chromatography, electrospray ionization mass spectrometry and gas chromatography/mass spectrometry. Phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, phosphatidylcholine and lyso-phosphatidylethanolamine were found in F. novicida. These glycerophospholipids contain fatty acids with broad range of chain lengths, and acyl chains have unusually different length in a single glycerophospholipid molecule. The special fatty acyl composition of glycerophospholipids in F. tularensis could potentially be used to detect the bacteria." @default.
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- W2105588400 date "2010-06-01" @default.
- W2105588400 modified "2023-10-17" @default.
- W2105588400 title "Application of electrospray ionization mass spectrometry to characterize glycerophospholipids in Francisella tularensis subsp. novicida" @default.
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- W2105588400 doi "https://doi.org/10.1016/j.ijms.2010.03.015" @default.
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