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- W2105603902 abstract "Light scattering is a long used non-invasive method to study biological cells. It is used extensively in flow cytometers, where light scattering by single cells is analyzed. However, solving the direct light scattering problem for single cells still presents lots of difficulties that are mainly caused by the size and shape of the cells. The size is in the range from a few wavelengths (for visible light) to several tens of wavelengths. This size range prohibits the use of long wavelength (Rayleigh) or short wavelength (Geometrical Optics (GO)) approximations. The shape in general is very complex with multiple inclusions, which can be smaller, comparable or considerably larger than the wavelength). This limits the use of quick, accurate techniques to very few cases. Internal inclusions with size comparable to wavelength also greatly complicate the application of GO even to very large cells. Another property of biological cells, which simplifies computations, is index-matching (when they are studied in liquid solution, as is usually the case). This property gives rise to several approximations, e.g. Rayleigh-Debye-Gans and anomalous diffraction, however they usually give satisfactory accuracy only to some measured quantities. Reference [1] is a recent review of light scattering by biological particles, where these issues are discussed in more detail. Although different new approximations are being developed, e.g. replacing certain cells by simpler shapes, validation of such approximations anyway require rigorous simulation of light scattering by complex-shaped particles. There are two types of methods, which are in principle capable of handling any geometry and size of the scatterers. They numerically solve the Maxwell equations in the time – Finite Difference Time Domain (FDTD) – or frequency – Discrete Dipole Approximation (DDA) – domain. Both methods divide the scatterer in subvolumes that should be much" @default.
- W2105603902 created "2016-06-24" @default.
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- W2105603902 date "2005-01-01" @default.
- W2105603902 modified "2023-09-23" @default.
- W2105603902 title "Capabilities of the discrete dipole approximation for simulation light scattering by biological cells" @default.
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