FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105642816> ?p ?o ?g. }

• W2105642816 endingPage "78" @default.
• W2105642816 startingPage "65" @default.
• W2105642816 abstract "The knowledge of oocyte activation and somatic cell nuclear transfer in the swamp buffalo (Buballus bubalis) is extremely rare. The objectives of this study were the following: (1) to investigate the various activation treatments on the parthenogenetic development of buffalo oocytes, (2) to examine the events of nuclear remodeling and in the in vitro development of cloned buffalo embryos reconstructed with serum fed or starved fetal fibroblasts, and (3) to investigate the in vivo development of cloned embryos derived from serum fed or starved cells after transfer into the recipients. The rates of cleavage and blastocyst development were found to be significantly higher (P < 0.05) when the oocytes were activated by the combination treatment of calcium ionophore (A23187) or ethanol followed by 6-DMAP than those activated by electrical pulses and 6-DMAP or other single treatments. Flow cytometric analysis revealed that the percentage in the G0/G1 phase in serum starved cells was significantly (P < 0.05) higher than that in serum fed cells (88.8 +/- 6.2 vs. 68.2 +/- 2.6). At 1 h post fusion (hpf), most of the transferred nuclei (71%) from serum fed cells did not change in size, and the nuclear envelope remained intact, whereas 29% underwent NEBD and PCC. When serum starved cells were used, 83% of the transferred nuclei underwent NEBD and PCC whereas 17% remained intact. The nuclear swelling and pronucleus (PN) formation were observed at 2-4 and 12 h post activation (hpa), respectively. The remodeled nuclei underwent mitotic division and developed to the 2-cell stage within 18-24 hpa. Fifty-five percent of oocytes reconstructed with serum fed cells were 2PN and 45% were 1PN, whereas 79% of the embryos reconstructed from starved cells were 1PN and 21% were 2PN. The percentage of blastocyst development of the embryos derived from starved cells was higher than that from the serum fed cells (35% vs. 21%, P < 0.05). Pregnancy was detected after the transfer of cloned blastocysts into the recipients but no recipients supported the development to term. The results of this work can be used to establish effective activation protocols for buffalo oocytes which can be used during nuclear transfer experiments." @default.
• W2105642816 created "2016-06-24" @default.
• W2105642816 creator A5002634330 @default.
• W2105642816 creator A5008811491 @default.
• W2105642816 creator A5012613418 @default.
• W2105642816 creator A5013963831 @default.
• W2105642816 creator A5034112861 @default.
• W2105642816 date "2004-01-01" @default.
• W2105642816 modified "2023-10-16" @default.
• W2105642816 title "Development of swamp buffalo (<i>Bubalus bubalis</i>) embryos after parthenogenetic activation and nuclear transfer using serum fed or starved fetal fibroblasts" @default.
• W2105642816 cites W1487014009 @default.
• W2105642816 cites W1585827177 @default.
• W2105642816 cites W177418593 @default.
• W2105642816 cites W1828591172 @default.
• W2105642816 cites W188087196 @default.
• W2105642816 cites W1916885428 @default.
• W2105642816 cites W1966131157 @default.
• W2105642816 cites W1977723116 @default.
• W2105642816 cites W1980395166 @default.
• W2105642816 cites W1993205220 @default.
• W2105642816 cites W2003701368 @default.
• W2105642816 cites W2005842414 @default.
• W2105642816 cites W2006492607 @default.
• W2105642816 cites W2007778824 @default.
• W2105642816 cites W2023149156 @default.
• W2105642816 cites W2024883340 @default.
• W2105642816 cites W2024926816 @default.
• W2105642816 cites W2027843778 @default.
• W2105642816 cites W2038718973 @default.
• W2105642816 cites W2046336403 @default.
• W2105642816 cites W2057071718 @default.
• W2105642816 cites W2063551001 @default.
• W2105642816 cites W2065784422 @default.
• W2105642816 cites W2066693111 @default.
• W2105642816 cites W2068196702 @default.
• W2105642816 cites W2074125341 @default.
• W2105642816 cites W2080737540 @default.
• W2105642816 cites W2081838669 @default.
• W2105642816 cites W2089626405 @default.
• W2105642816 cites W2106509794 @default.
• W2105642816 cites W2108949541 @default.
• W2105642816 cites W2112233726 @default.
• W2105642816 cites W2115713113 @default.
• W2105642816 cites W2132706361 @default.
• W2105642816 cites W2136379538 @default.
• W2105642816 cites W2146124982 @default.
• W2105642816 cites W2149346011 @default.
• W2105642816 cites W2151637761 @default.
• W2105642816 cites W2156139452 @default.
• W2105642816 cites W2161298317 @default.
• W2105642816 cites W2394664013 @default.
• W2105642816 doi "https://doi.org/10.1051/rnd:2004017" @default.
• W2105642816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15189011" @default.
• W2105642816 hasPublicationYear "2004" @default.
• W2105642816 type Work @default.
• W2105642816 sameAs 2105642816 @default.
• W2105642816 citedByCount "40" @default.
• W2105642816 countsByYear W21056428162012 @default.
• W2105642816 countsByYear W21056428162013 @default.
• W2105642816 countsByYear W21056428162014 @default.
• W2105642816 countsByYear W21056428162015 @default.
• W2105642816 countsByYear W21056428162017 @default.
• W2105642816 countsByYear W21056428162018 @default.
• W2105642816 countsByYear W21056428162020 @default.
• W2105642816 countsByYear W21056428162021 @default.
• W2105642816 countsByYear W21056428162022 @default.
• W2105642816 crossrefType "journal-article" @default.
• W2105642816 hasAuthorship W2105642816A5002634330 @default.
• W2105642816 hasAuthorship W2105642816A5008811491 @default.
• W2105642816 hasAuthorship W2105642816A5012613418 @default.
• W2105642816 hasAuthorship W2105642816A5013963831 @default.
• W2105642816 hasAuthorship W2105642816A5034112861 @default.
• W2105642816 hasBestOaLocation W21056428161 @default.
• W2105642816 hasConcept C103725303 @default.
• W2105642816 hasConcept C104317684 @default.
• W2105642816 hasConcept C110758877 @default.
• W2105642816 hasConcept C134305767 @default.
• W2105642816 hasConcept C153911025 @default.
• W2105642816 hasConcept C160099875 @default.
• W2105642816 hasConcept C16685009 @default.
• W2105642816 hasConcept C172680121 @default.
• W2105642816 hasConcept C179265757 @default.
• W2105642816 hasConcept C196843134 @default.
• W2105642816 hasConcept C202438428 @default.
• W2105642816 hasConcept C2778177303 @default.
• W2105642816 hasConcept C2779234561 @default.
• W2105642816 hasConcept C28748434 @default.
• W2105642816 hasConcept C54355233 @default.
• W2105642816 hasConcept C55493867 @default.
• W2105642816 hasConcept C71924100 @default.
• W2105642816 hasConcept C86803240 @default.
• W2105642816 hasConcept C87073359 @default.
• W2105642816 hasConcept C93304396 @default.
• W2105642816 hasConcept C95444343 @default.
• W2105642816 hasConceptScore W2105642816C103725303 @default.
• W2105642816 hasConceptScore W2105642816C104317684 @default.
• W2105642816 hasConceptScore W2105642816C110758877 @default.
• W2105642816 hasConceptScore W2105642816C134305767 @default.

• next