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• W2105698514 abstract "ABSTRACT Mutants blocked at the earliest stage of morphological development in Streptomyces species are called bld mutants. These mutants are pleiotropically defective in the initiation of development, the ability to produce antibiotics, the ability to regulate carbon utilization, and the ability to send and/or respond to extracellular signals. Here we report the identification and partial characterization of a 99-amino-acid open reading frame (ORF99) that is capable of restoring morphogenesis, antibiotic production, and catabolite control to all of the bldB mutants. Of the existing bld mutants, bldB is of special interest because the phenotype of this mutant is the most pleiotropic. DNA sequence analysis of ORF99 from each of the existing bldB mutants identified base changes either within the coding region of the predicted protein or in the regulatory region of the gene. Primer extension analysis identified an apparent transcription start site. A promoter fusion to the xylE reporter gene showed that expression of bldB is apparently temporally regulated and that the bldB gene product is involved in the regulation of its own expression." @default.
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• W2105698514 date "1998-03-15" @default.
• W2105698514 modified "2023-10-12" @default.
• W2105698514 title "The <i>bldB</i> Gene Encodes a Small Protein Required for Morphogenesis, Antibiotic Production, and Catabolite Control in <i>Streptomyces coelicolor</i>" @default.
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• W2105698514 doi "https://doi.org/10.1128/jb.180.6.1556-1562.1998" @default.
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