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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105718057> ?p ?o ?g. }

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• W2105718057 startingPage "3367" @default.
• W2105718057 abstract "A fluorescence polarization assay is described that measures the binding of fluorescently labeled erythromycin to 70S ribosomes from Escherichia coli and the displacement of the erythromycin from these ribosomes. The assay has been validated with several macrolide derivatives and other known antibiotics. We demonstrate that this assay is suitable for determining the dissociation constants of novel compounds that have binding sites overlapping those of macrolides. This homogeneous binding assay provides a valuable tool for defining structure-activity relationships among compounds during the discovery and development of new ribosome-targeting drugs." @default.
• W2105718057 created "2016-06-24" @default.
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• W2105718057 date "2005-08-01" @default.
• W2105718057 modified "2023-10-14" @default.
• W2105718057 title "Fluorescence Polarization Method To Characterize Macrolide-Ribosome Interactions" @default.
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• W2105718057 doi "https://doi.org/10.1128/aac.49.8.3367-3372.2005" @default.
• W2105718057 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1196252" @default.
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