FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105721076> ?p ?o ?g. }

• W2105721076 endingPage "5587" @default.
• W2105721076 startingPage "5580" @default.
• W2105721076 abstract "ABSTRACT During infection of Escherichia coli , bacteriophage T4 usurps the host transcriptional machinery, redirecting it to the expression of early, middle, and late phage genes. Middle genes, whose expression begins about 1 min postinfection, are transcribed both from the extension of early RNA into middle genes and by the activation of T4 middle promoters. Middle-promoter activation requires the T4 transcriptional activator MotA and coactivator AsiA, which are known to interact with σ 70 , the specificity subunit of RNA polymerase. T4 motA amber [ motA (Am)] or asiA (Am) phage grows poorly in wild-type E. coli . However, previous work has found that T4 motA (Am)does not grow in the E. coli mutant strain TabG. We show here that the RNA polymerase in TabG contains two mutations within its β-subunit gene: rpoB ( E835K ) and rpoB ( G1249D ). We find that the G1249D mutation is responsible for restricting the growth of either T4 motA (Am)or asiA (Am) and for impairing transcription from MotA/AsiA-activated middle promoters in vivo . With one exception, transcription from tested T4 early promoters is either unaffected or, in some cases, even increases, and there is no significant growth phenotype for the rpoB ( E835K G1249D ) strain in the absence of T4 infection. In reported structures of thermophilic RNA polymerase, the G1249 residue is located immediately adjacent to a hydrophobic pocket, called the switch 3 loop. This loop is thought to aid in the separation of the RNA from the DNA-RNA hybrid as RNA enters the RNA exit channel. Our results suggest that the presence of MotA and AsiA may impair the function of this loop or that this portion of the β subunit may influence interactions among MotA, AsiA, and RNA polymerase." @default.
• W2105721076 created "2016-06-24" @default.
• W2105721076 creator A5008258237 @default.
• W2105721076 creator A5029069211 @default.
• W2105721076 creator A5045012027 @default.
• W2105721076 date "2010-11-01" @default.
• W2105721076 modified "2023-09-27" @default.
• W2105721076 title "A Mutation within the β Subunit of <i>Escherichia coli</i> RNA Polymerase Impairs Transcription from Bacteriophage T4 Middle Promoters" @default.
• W2105721076 cites W1508617114 @default.
• W2105721076 cites W1516828376 @default.
• W2105721076 cites W1518350841 @default.
• W2105721076 cites W1543804839 @default.
• W2105721076 cites W1578016329 @default.
• W2105721076 cites W1587481278 @default.
• W2105721076 cites W1602440978 @default.
• W2105721076 cites W1603424960 @default.
• W2105721076 cites W1784080967 @default.
• W2105721076 cites W1816925967 @default.
• W2105721076 cites W1873199501 @default.
• W2105721076 cites W1963558018 @default.
• W2105721076 cites W1963806581 @default.
• W2105721076 cites W1967514004 @default.
• W2105721076 cites W1969906365 @default.
• W2105721076 cites W1970596604 @default.
• W2105721076 cites W1971100508 @default.
• W2105721076 cites W1979052823 @default.
• W2105721076 cites W1981112331 @default.
• W2105721076 cites W1981374436 @default.
• W2105721076 cites W1985229621 @default.
• W2105721076 cites W1985301420 @default.
• W2105721076 cites W1989380134 @default.
• W2105721076 cites W1994330097 @default.
• W2105721076 cites W1997268928 @default.
• W2105721076 cites W2006373999 @default.
• W2105721076 cites W2009659277 @default.
• W2105721076 cites W2010742082 @default.
• W2105721076 cites W2012256530 @default.
• W2105721076 cites W2034880435 @default.
• W2105721076 cites W2038059597 @default.
• W2105721076 cites W2042586799 @default.
• W2105721076 cites W2042596462 @default.
• W2105721076 cites W2043098161 @default.
• W2105721076 cites W2045902027 @default.
• W2105721076 cites W2047611481 @default.
• W2105721076 cites W2058361908 @default.
• W2105721076 cites W2065310225 @default.
• W2105721076 cites W2065930935 @default.
• W2105721076 cites W2075743405 @default.
• W2105721076 cites W2086269772 @default.
• W2105721076 cites W2087447141 @default.
• W2105721076 cites W2089631335 @default.
• W2105721076 cites W2092682318 @default.
• W2105721076 cites W2094042154 @default.
• W2105721076 cites W2113938608 @default.
• W2105721076 cites W2127848647 @default.
• W2105721076 cites W2147057508 @default.
• W2105721076 cites W2159491669 @default.
• W2105721076 cites W2160521062 @default.
• W2105721076 cites W2168597324 @default.
• W2105721076 doi "https://doi.org/10.1128/jb.00338-10" @default.
• W2105721076 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2953677" @default.
• W2105721076 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20729353" @default.
• W2105721076 hasPublicationYear "2010" @default.
• W2105721076 type Work @default.
• W2105721076 sameAs 2105721076 @default.
• W2105721076 citedByCount "13" @default.
• W2105721076 countsByYear W21057210762012 @default.
• W2105721076 countsByYear W21057210762014 @default.
• W2105721076 countsByYear W21057210762016 @default.
• W2105721076 countsByYear W21057210762017 @default.
• W2105721076 countsByYear W21057210762018 @default.
• W2105721076 countsByYear W21057210762019 @default.
• W2105721076 countsByYear W21057210762020 @default.
• W2105721076 countsByYear W21057210762021 @default.
• W2105721076 crossrefType "journal-article" @default.
• W2105721076 hasAuthorship W2105721076A5008258237 @default.
• W2105721076 hasAuthorship W2105721076A5029069211 @default.
• W2105721076 hasAuthorship W2105721076A5045012027 @default.
• W2105721076 hasBestOaLocation W21057210761 @default.
• W2105721076 hasConcept C101762097 @default.
• W2105721076 hasConcept C104317684 @default.
• W2105721076 hasConcept C138885662 @default.
• W2105721076 hasConcept C150194340 @default.
• W2105721076 hasConcept C153911025 @default.
• W2105721076 hasConcept C172768829 @default.
• W2105721076 hasConcept C179926584 @default.
• W2105721076 hasConcept C25281209 @default.
• W2105721076 hasConcept C2776441376 @default.
• W2105721076 hasConcept C2776449523 @default.
• W2105721076 hasConcept C2778340795 @default.
• W2105721076 hasConcept C41258723 @default.
• W2105721076 hasConcept C41895202 @default.
• W2105721076 hasConcept C42062724 @default.
• W2105721076 hasConcept C47319516 @default.
• W2105721076 hasConcept C54355233 @default.
• W2105721076 hasConcept C547475151 @default.
• W2105721076 hasConcept C64350747 @default.
• W2105721076 hasConcept C67705224 @default.

• next