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- W2105751329 abstract "Chlorides with different paramagnetic cations such as Ni2+, Co2+ and Mn2+ were used as crystallizing agents instead of NaCl to crystallize hen egg-white lysozyme. NiCl2 was found to give two types of crystals with different morphologies: one (roof-like) is a new type of orthorhombic P212121 crystal with lattice constants a=79.0 Å, b=80.8 Å, and c=37.5 Å; the second is an ordinary tetragonal crystal of its characteristic shape with a=b=80 Å and c=38 Å. The appearance of the roof-like shape became dominant in the presence of a magnetic field. In the case of using CoCl2 and MnCl2, ordinary tetragonal crystals were formed. A striking fact was that the a-axis of the crystals oriented along the magnetic field when CoCl2 was used, as opposed to the usual c-axis orientation. Large and optically perfect lysozyme crystals can be obtained in a magnetic field when NiCl2 or MnCl2 is used as a crystallizing agent. These profound effects of the paramagnetic cations may be caused by the coordination of Ni2+ and Co2+ ions to a lysozyme molecule, which was found by X-ray crystallography." @default.
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- W2105751329 date "2003-05-01" @default.
- W2105751329 modified "2023-10-18" @default.
- W2105751329 title "New morphology, symmetry, orientation and perfection of lysozyme crystals grown in a magnetic field when paramagnetic salts (NiCl2, CoCl2 and MnCl2) are used as crystallizing agents" @default.
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- W2105751329 doi "https://doi.org/10.1016/s0022-0248(03)00952-7" @default.
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