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- W2105806616 abstract "The N-methyl-D-aspartate receptor (NMDAR) is a member of the glutamate receptor family of proteins and is responsible for excitatory transmission. Activation of the receptor is thought to be controlled by conformational changes in the ligand binding domain (LBD); however, glutamate receptor LBDs can occupy multiple conformations even in the activated form. This work probes equilibrium transitions among NMDAR LBD conformations by monitoring the distance across the glycine-bound LBD cleft using single-molecule Förster resonance energy transfer (smFRET). Recent improvements in photoprotection solutions allowed us to monitor transitions among the multiple conformations. Also, we applied a recently developed model-free algorithm called step transition and state identification to identify the number of states, their smFRET efficiencies, and their interstate kinetics. Reversible interstate conversions, corresponding to transitions among a wide range of cleft widths, were identified in the glycine-bound LBD, on much longer timescales compared to channel opening. These transitions were confirmed to be equilibrium in nature by shifting the distribution reversibly via denaturant. We found that the NMDAR LBD proceeds primarily from one adjacent smFRET state to the next under equilibrium conditions, consistent with a cleft-opening/closing mechanism. Overall, by analyzing the state-to-state transition dynamics and distributions, we achieve insight into specifics of long-lived LBD equilibrium structural dynamics, as well as obtain a more general description of equilibrium folding/unfolding in a conformationally dynamic protein. The relationship between such long-lived LBD dynamics and channel function in the full receptor remains an open and interesting question." @default.
- W2105806616 created "2016-06-24" @default.
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- W2105806616 date "2015-07-01" @default.
- W2105806616 modified "2023-10-17" @default.
- W2105806616 title "Conformational Transitions in the Glycine-Bound GluN1 NMDA Receptor LBD via Single-Molecule FRET" @default.
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- W2105806616 cites W1976966398 @default.
- W2105806616 cites W1978558648 @default.
- W2105806616 cites W1978958559 @default.
- W2105806616 cites W1981708871 @default.
- W2105806616 cites W1984865310 @default.
- W2105806616 cites W1986826865 @default.
- W2105806616 cites W1990567099 @default.
- W2105806616 cites W1995852331 @default.
- W2105806616 cites W1999644112 @default.
- W2105806616 cites W2001402270 @default.
- W2105806616 cites W2004670135 @default.
- W2105806616 cites W2009627426 @default.
- W2105806616 cites W2010926499 @default.
- W2105806616 cites W2011256514 @default.
- W2105806616 cites W2012641732 @default.
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- W2105806616 cites W2026606828 @default.
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- W2105806616 cites W2037852973 @default.
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- W2105806616 cites W2043736118 @default.
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- W2105806616 cites W2054953297 @default.
- W2105806616 cites W2055264837 @default.
- W2105806616 cites W2064173101 @default.
- W2105806616 cites W2064954550 @default.
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- W2105806616 cites W2075494942 @default.
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- W2105806616 cites W2082888427 @default.
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- W2105806616 cites W2089094370 @default.
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- W2105806616 doi "https://doi.org/10.1016/j.bpj.2015.05.025" @default.
- W2105806616 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4572502" @default.
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- W2105806616 hasPublicationYear "2015" @default.
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