Matches in SemOpenAlex for { <https://semopenalex.org/work/W2105815346> ?p ?o ?g. }
- W2105815346 endingPage "8737" @default.
- W2105815346 startingPage "8726" @default.
- W2105815346 abstract "Synthetic acid tolerance, especially during active cell growth, is a desirable phenotype for many biotechnological applications. Natively, acid resistance in Escherichia coli is largely a stationary-phase phenotype attributable to mechanisms mostly under the control of the stationary-phase sigma factor RpoS. We show that simultaneous overexpression of noncoding small RNAs (sRNAs), DsrA, RprA and ArcZ, which are translational RpoS activators, increased acid tolerance (based on a low-pH survival assay) supra-additively up to 8500-fold during active cell growth, and provided protection against carboxylic acid and oxidative stress. Overexpression of rpoS without its regulatory 5′-UTR resulted in inferior acid tolerance. The supra-additive effect of overexpressing the three sRNAs results from the impact their expression has on RpoS-protein levels, and the beneficial perturbation of the interconnected RpoS and H-NS networks, thus leading to superior tolerance during active growth. Unlike the overexpression of proteins, overexpression of sRNAs imposes hardly any metabolic burden on cells, and constitutes a more effective strain engineering strategy." @default.
- W2105815346 created "2016-06-24" @default.
- W2105815346 creator A5012390434 @default.
- W2105815346 creator A5041370143 @default.
- W2105815346 creator A5041980910 @default.
- W2105815346 creator A5054297565 @default.
- W2105815346 creator A5059340897 @default.
- W2105815346 date "2013-07-27" @default.
- W2105815346 modified "2023-09-23" @default.
- W2105815346 title "Synthetic tolerance: three noncoding small RNAs, DsrA, ArcZ and RprA, acting supra-additively against acid stress" @default.
- W2105815346 cites W1526440925 @default.
- W2105815346 cites W1551233459 @default.
- W2105815346 cites W1556986896 @default.
- W2105815346 cites W1574886161 @default.
- W2105815346 cites W1578941595 @default.
- W2105815346 cites W1624096210 @default.
- W2105815346 cites W1691891364 @default.
- W2105815346 cites W1965942481 @default.
- W2105815346 cites W1970677041 @default.
- W2105815346 cites W1986555537 @default.
- W2105815346 cites W1987573660 @default.
- W2105815346 cites W1994508208 @default.
- W2105815346 cites W1999305069 @default.
- W2105815346 cites W2000017565 @default.
- W2105815346 cites W2001061405 @default.
- W2105815346 cites W2004254961 @default.
- W2105815346 cites W2010290489 @default.
- W2105815346 cites W2017522869 @default.
- W2105815346 cites W2018775916 @default.
- W2105815346 cites W2022126733 @default.
- W2105815346 cites W2023220522 @default.
- W2105815346 cites W2032582595 @default.
- W2105815346 cites W2038613897 @default.
- W2105815346 cites W2053138695 @default.
- W2105815346 cites W2058021523 @default.
- W2105815346 cites W2063555866 @default.
- W2105815346 cites W2069698199 @default.
- W2105815346 cites W2070456506 @default.
- W2105815346 cites W2073638945 @default.
- W2105815346 cites W2096046927 @default.
- W2105815346 cites W2100737694 @default.
- W2105815346 cites W2102263295 @default.
- W2105815346 cites W2105133080 @default.
- W2105815346 cites W2107277218 @default.
- W2105815346 cites W2110528780 @default.
- W2105815346 cites W2114448039 @default.
- W2105815346 cites W2123589399 @default.
- W2105815346 cites W2124765964 @default.
- W2105815346 cites W2129162353 @default.
- W2105815346 cites W2134300559 @default.
- W2105815346 cites W2136072677 @default.
- W2105815346 cites W2142136966 @default.
- W2105815346 cites W2144458042 @default.
- W2105815346 cites W2147853672 @default.
- W2105815346 cites W2148382830 @default.
- W2105815346 cites W2148885731 @default.
- W2105815346 cites W2149699755 @default.
- W2105815346 cites W2152232041 @default.
- W2105815346 cites W2154488487 @default.
- W2105815346 cites W2155297184 @default.
- W2105815346 cites W2156522711 @default.
- W2105815346 cites W2158408911 @default.
- W2105815346 cites W2165202736 @default.
- W2105815346 cites W2168989431 @default.
- W2105815346 cites W2170360177 @default.
- W2105815346 doi "https://doi.org/10.1093/nar/gkt651" @default.
- W2105815346 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3794604" @default.
- W2105815346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23892399" @default.
- W2105815346 hasPublicationYear "2013" @default.
- W2105815346 type Work @default.
- W2105815346 sameAs 2105815346 @default.
- W2105815346 citedByCount "88" @default.
- W2105815346 countsByYear W21058153462013 @default.
- W2105815346 countsByYear W21058153462014 @default.
- W2105815346 countsByYear W21058153462015 @default.
- W2105815346 countsByYear W21058153462016 @default.
- W2105815346 countsByYear W21058153462017 @default.
- W2105815346 countsByYear W21058153462018 @default.
- W2105815346 countsByYear W21058153462019 @default.
- W2105815346 countsByYear W21058153462020 @default.
- W2105815346 countsByYear W21058153462021 @default.
- W2105815346 countsByYear W21058153462022 @default.
- W2105815346 countsByYear W21058153462023 @default.
- W2105815346 crossrefType "journal-article" @default.
- W2105815346 hasAuthorship W2105815346A5012390434 @default.
- W2105815346 hasAuthorship W2105815346A5041370143 @default.
- W2105815346 hasAuthorship W2105815346A5041980910 @default.
- W2105815346 hasAuthorship W2105815346A5054297565 @default.
- W2105815346 hasAuthorship W2105815346A5059340897 @default.
- W2105815346 hasBestOaLocation W21058153462 @default.
- W2105815346 hasConcept C101762097 @default.
- W2105815346 hasConcept C104317684 @default.
- W2105815346 hasConcept C105580179 @default.
- W2105815346 hasConcept C127716648 @default.
- W2105815346 hasConcept C149364088 @default.
- W2105815346 hasConcept C150194340 @default.
- W2105815346 hasConcept C185592680 @default.
- W2105815346 hasConcept C2776449523 @default.