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• W2105852372 abstract "ABSTRACT Vibrio parahaemolyticus is a halophilic bacterium frequently involved in human outbreaks of seafood-associated gastroenteritis. For epidemiological purposes, different molecular typing methods, such as pulsed-field gel electrophoresis (PFGE) or ribotyping, have been developed for this pathogen; however, these methods are mostly labor-intensive and time-consuming. In this work, we designed and evaluated three rapid PCR typing methods for this pathogen using primers designed on the basis of the following specific sequences: conserved ribosomal gene spacer sequence (RS), repetitive extragenic palindromic sequence (REP), and enterobacterial repetitive intergenic consensus sequence (ERIC). Typing patterns and clustering analysis indicated that these methods apparently differentiated V . parahaemolyticus strains from reference strains of interspecific Escherichia coli , V . cholerae , and V . vulnificus and were also valuable in subspecies typing of this pathogen. Forty domestic strains of V . parahaemolyticus , representing a wide range of PFGE patterns, were grouped into 15, 27, and 27 patterns, with discrimination indexes of 0.91, 0.97, and 0.98, by RS-, REP-, and ERIC-PCR, respectively. The discriminative abilities of these PCR methods closely approached or even exceeded those of PFGE and ribotyping. REP-PCR is preferable to ERIC-PCR because of the greater reproducibility of its fingerprints, while RS-PCR may be a practical method because it generates fewer amplification bands and patterns than the alternatives." @default.
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• W2105852372 date "2001-12-01" @default.
• W2105852372 modified "2023-10-11" @default.
• W2105852372 title "Evaluation of Typing of <i>Vibrio</i> <i>parahaemolyticus</i> by Three PCR Methods Using Specific Primers" @default.
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• W2105852372 doi "https://doi.org/10.1128/jcm.39.12.4233-4240.2001" @default.
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