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• W2105876504 abstract "The biological aromatization of androstenedione-4-C 14 , testosterone-4-C 14 , and dehydroepiandrosterone-4-C 14 by surviving human normal and Stein–Leventhal-type ovarian slices was investigated. The precursors were incubated with the tissue slices in a Krebs–Ringer–phosphate–glucose medium (pH 7.4) in an O 2 atmosphere at 37 °C for 6 and (or) 24 hours. As a cofactor, 1000 I.U. of human chorionic gonadotrophin was added to each incubation vessel. Both normal and polymicrocystic ovarian slices transformed the precursors partially to estradiol-17β. This compound was identified by isotopic dilution techniques and by the constancy of specific activities of its chemical derivatives. There was no significant qualitative or quantitative difference in the aromatization capacity of the two kinds of tissue. In addition, biosynthetic androstenedione-4-C 14 and testo-sterone-4-C 14 were positively identified. These experiments seem to indicate that under the experimental conditions used, the aromatization mechanism is functioning normally in Stein–Leventhal-type ovaries." @default.
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• W2105876504 date "1963-03-01" @default.
• W2105876504 modified "2023-10-03" @default.
• W2105876504 title "THE IN VITRO BIOSYNTHESIS OF ESTRADIOL-17β-4-C¹⁴BY SURVIVING NORMAL AND STEIN–LEVENTHAL-TYPE OVARIAN SLICES" @default.
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• W2105876504 doi "https://doi.org/10.1139/o63-076" @default.
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