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• W2105938928 startingPage "1415" @default.
• W2105938928 abstract "The nucleobase modification 5-methylcytosine (m5C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m5C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modified nucleobase in RNA still remain to be elucidated. The recent development of a bisulfite sequencing approach for efficient m5C localization in various RNA molecules puts ribo-m5C in a highly privileged position as one of the few RNA modifications whose detection is amenable to PCR-based amplification and sequencing methods. Additional progress in the field also includes the characterization of several specific RNA methyltransferase enzymes in various organisms, and the discovery of a new and unexpected link between DNA and RNA m5C-methylation. Numerous putative RNA:m5C-MTases have now been identified and are awaiting characterization, including the identification of their RNA substrates and their related cellular functions. In order to bring these recent exciting developments into perspective, this review provides an ordered overview of the detection methods for RNA methylation, of the biochemistry, enzymology and molecular biology of the corresponding modification enzymes, and discusses perspectives for the emerging biological functions of these enzymes." @default.
• W2105938928 created "2016-06-24" @default.
• W2105938928 creator A5001477363 @default.
• W2105938928 creator A5052604481 @default.
• W2105938928 creator A5089150142 @default.
• W2105938928 date "2009-12-08" @default.
• W2105938928 modified "2023-10-11" @default.
• W2105938928 title "5-methylcytosine in RNA: detection, enzymatic formation and biological functions" @default.
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