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• W2105947527 abstract "Hepatic metastasis is a primary cause for failure of locoregional therapy in colorectal cancer. Increased expression of osteopontin (OPN), a ligand for α v β 3 integrin and CD44 receptors, is associated with metastasis in several types of cancer. However, the mechanism by which OPN mediates metastasis in colorectal cancer remains unknown. We hypothesized that OPN mediates invasion of colon cancer cells through basement membrane and migration through extracellular matrix (ECM). In this study, we used CT26 murine colon adenocarcinoma cells syngeneic to BALB/c mice to generate cell lines (pS-OPN) in which OPN expression was suppressed through small interfering RNA (siRNA) plasmids. CT26 wild-type cells (WT) and CT26 cells stably expressing murine-mismatch siRNA (pS-MM) served as controls. Western blotting quantified OPN protein levels and our most downregulated clone, pS-OPN-A4, demonstrated a mean 3.0-fold decrease in OPN protein expression versus WT. In vitro cell motility and invasiveness were decreased in pS-OPN-A4 by 3.6-fold ( P = 0.004 versus WT) and 4.1-fold ( P = 0.01 versus WT), but proliferation was similar amongst cell lines. We demonstrated that OPN suppression significantly correlates with MMP-2 downregulation. In vivo hepatic metastasis was assessed by quantifying liver weights and surface tumor nodules in 33 BALB/c mice (11/group) subjected to intrasplenic injection of tumor cells. pS-OPN-A4 resulted in a 50.4% decrease in mean liver weight compared with WT (3.79 ± 1.49 g versus 1.88 ± 1.34 g, P = 0.009). Only 18% of pS-OPN-A4 livers had >20 metastatic surface nodules compared with 89% for WT and 75% for pS-MM-V6. This study demonstrates that RNA interference stably reduces CT26 tumor expression of OPN and significantly attenuates CT26 colon cancer metastasis by diminishing tumor cell motility and invasiveness." @default.
• W2105947527 created "2016-06-24" @default.
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• W2105947527 date "2005-01-06" @default.
• W2105947527 modified "2023-10-12" @default.
• W2105947527 title "Osteopontin silencing by small interfering RNA suppresses in vitro and in vivo CT26 murine colon adenocarcinoma metastasis" @default.
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• W2105947527 cites W1530946237 @default.
• W2105947527 cites W1533127962 @default.
• W2105947527 cites W1534278651 @default.
• W2105947527 cites W1611419331 @default.
• W2105947527 cites W1615967354 @default.
• W2105947527 cites W1716523897 @default.
• W2105947527 cites W1740440689 @default.
• W2105947527 cites W1784982965 @default.
• W2105947527 cites W1888555575 @default.
• W2105947527 cites W1910325808 @default.
• W2105947527 cites W1926596361 @default.
• W2105947527 cites W1939982746 @default.
• W2105947527 cites W1963555207 @default.
• W2105947527 cites W1964887530 @default.
• W2105947527 cites W1972162901 @default.
• W2105947527 cites W1972959987 @default.
• W2105947527 cites W1974208182 @default.
• W2105947527 cites W1974406951 @default.
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• W2105947527 cites W1993073412 @default.
• W2105947527 cites W2006877529 @default.
• W2105947527 cites W2010690155 @default.
• W2105947527 cites W2012731291 @default.
• W2105947527 cites W2018235962 @default.
• W2105947527 cites W2019390346 @default.
• W2105947527 cites W2020392843 @default.
• W2105947527 cites W2033903142 @default.
• W2105947527 cites W2033985429 @default.
• W2105947527 cites W2037100155 @default.
• W2105947527 cites W2042346313 @default.
• W2105947527 cites W2048457753 @default.
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• W2105947527 cites W2077569218 @default.
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• W2105947527 cites W2170020426 @default.
• W2105947527 cites W2319411522 @default.
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• W2105947527 doi "https://doi.org/10.1093/carcin/bgi027" @default.
• W2105947527 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15661802" @default.
• W2105947527 hasPublicationYear "2005" @default.
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