FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2106043220> ?p ?o ?g. }

• W2106043220 endingPage "381" @default.
• W2106043220 startingPage "371" @default.
• W2106043220 abstract "1. The isoform-specific functional role of cytoplasmic structures of two voltage-gated sodium channel isoforms, the human cardiac channel (hH1) and the adult human skeletal muscle channel (hSkM1) was investigated through functional comparison of chimeras. 2. The voltage of half-activation (V(a)) for hH1 was shifted by > 20 mV in the hyperpolarised direction following internal papain treatment ('papain sensitive'), while V(a) for hSkM1 was unaffected ('papain insensitive'). 3. The hH1 region(s) responsible for this papain sensitivity was localised by testing a series of hH1/hSkM1 chimeras in which combinations of the large hH1 cytoplasmic loops joining the four transmembrane domains replaced analogous hSkM1 loops. Various chimeras were used to determine the smallest subset of loops that converted fully the papain-insensitive hSkM1 into a papain-sensitive channel. Then three converse chimeras were tested in which hSkM1 loops replaced hH1 loops to determine the smallest subset of loops necessary and sufficient to convert the papain-sensitive hH1 into a papain-insensitive channel. 4. Functional studies of this inclusive set of chimeras indicate that the first two cytoplasmic loops of the cardiac sodium channel that join domain I to II (loop A), and domain II to III (loop B), are both necessary, and together are sufficient to produce a papain-induced hyperpolarising shift in the voltage at which channels activate. When both loops are present (wild-type hH1 and the chimera hSkM1AB), V(a) for the channel shifts in the hyperpolarised direction by > 20 mV with papain treatment. When the analogous hSkM1 loops are present (wild-type hSkM1 and the chimera hH1AB), V(a) for the channel is not sensitive to treatment with papain. For channels that contain only one of the two hH1 loops, the effect of papain on V(a) is intermediary. 5. Experiments performed in the absence of papain showed that the activation voltages of the double loop chimeras, hSkM1AB and hH1AB, were shifted significantly from V(a) for hSkM1 and V(a) for hH1, respectively, indicating that these loops directly alter channel activation voltage. The resulting shifts in V(a) were in opposing directions, suggesting that cytoplasmic control of activation voltage is isoform specific. V(a) for hSkM1AB was about 20 mV more depolarised than V(a) for hSkM1, and V(a) for hH1AB was about 9 mV more negative than V(a) for hH1. 6. These data are the first to indicate isoform-specific cytoplasmic regions of the voltage-gated sodium channel that directly and differently alter the voltage of channel activation." @default.
• W2106043220 created "2016-06-24" @default.
• W2106043220 creator A5061261292 @default.
• W2106043220 date "2001-09-01" @default.
• W2106043220 modified "2023-10-18" @default.
• W2106043220 title "Channel cytoplasmic loops alter voltage‐dependent sodium channel activation in an isoform‐specific manner" @default.
• W2106043220 cites W1792349030 @default.
• W2106043220 cites W1963588589 @default.
• W2106043220 cites W1966547075 @default.
• W2106043220 cites W1973420297 @default.
• W2106043220 cites W1975531408 @default.
• W2106043220 cites W1985150523 @default.
• W2106043220 cites W1986785675 @default.
• W2106043220 cites W1998006950 @default.
• W2106043220 cites W1998457528 @default.
• W2106043220 cites W2001539229 @default.
• W2106043220 cites W2026472308 @default.
• W2106043220 cites W2027786686 @default.
• W2106043220 cites W2042089391 @default.
• W2106043220 cites W2043076822 @default.
• W2106043220 cites W2044076590 @default.
• W2106043220 cites W2051237605 @default.
• W2106043220 cites W2054227438 @default.
• W2106043220 cites W2055468934 @default.
• W2106043220 cites W2067576287 @default.
• W2106043220 cites W2068485512 @default.
• W2106043220 cites W2081880350 @default.
• W2106043220 cites W2085873341 @default.
• W2106043220 cites W2090038598 @default.
• W2106043220 cites W2092351084 @default.
• W2106043220 cites W2111066407 @default.
• W2106043220 cites W2111853402 @default.
• W2106043220 cites W2113398296 @default.
• W2106043220 cites W2114161133 @default.
• W2106043220 cites W2118068129 @default.
• W2106043220 cites W2119278180 @default.
• W2106043220 cites W2125875893 @default.
• W2106043220 cites W2126490502 @default.
• W2106043220 cites W2129747642 @default.
• W2106043220 cites W2130972481 @default.
• W2106043220 cites W2131381195 @default.
• W2106043220 cites W2132651808 @default.
• W2106043220 cites W2143319028 @default.
• W2106043220 cites W2155979828 @default.
• W2106043220 cites W2159340418 @default.
• W2106043220 cites W2171411680 @default.
• W2106043220 cites W2172530119 @default.
• W2106043220 cites W2267906346 @default.
• W2106043220 cites W2332764748 @default.
• W2106043220 cites W2406721579 @default.
• W2106043220 cites W4252179992 @default.
• W2106043220 doi "https://doi.org/10.1111/j.1469-7793.2001.t01-1-00371.x" @default.
• W2106043220 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2278789" @default.
• W2106043220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11533130" @default.
• W2106043220 hasPublicationYear "2001" @default.
• W2106043220 type Work @default.
• W2106043220 sameAs 2106043220 @default.
• W2106043220 citedByCount "14" @default.
• W2106043220 countsByYear W21060432202015 @default.
• W2106043220 countsByYear W21060432202020 @default.
• W2106043220 crossrefType "journal-article" @default.
• W2106043220 hasAuthorship W2106043220A5061261292 @default.
• W2106043220 hasBestOaLocation W21060432202 @default.
• W2106043220 hasConcept C104317684 @default.
• W2106043220 hasConcept C118892022 @default.
• W2106043220 hasConcept C12554922 @default.
• W2106043220 hasConcept C170493617 @default.
• W2106043220 hasConcept C178790620 @default.
• W2106043220 hasConcept C181199279 @default.
• W2106043220 hasConcept C185592680 @default.
• W2106043220 hasConcept C190062978 @default.
• W2106043220 hasConcept C24530287 @default.
• W2106043220 hasConcept C2779832904 @default.
• W2106043220 hasConcept C41625074 @default.
• W2106043220 hasConcept C50952357 @default.
• W2106043220 hasConcept C53345823 @default.
• W2106043220 hasConcept C537181965 @default.
• W2106043220 hasConcept C55493867 @default.
• W2106043220 hasConcept C86803240 @default.
• W2106043220 hasConceptScore W2106043220C104317684 @default.
• W2106043220 hasConceptScore W2106043220C118892022 @default.
• W2106043220 hasConceptScore W2106043220C12554922 @default.
• W2106043220 hasConceptScore W2106043220C170493617 @default.
• W2106043220 hasConceptScore W2106043220C178790620 @default.
• W2106043220 hasConceptScore W2106043220C181199279 @default.
• W2106043220 hasConceptScore W2106043220C185592680 @default.
• W2106043220 hasConceptScore W2106043220C190062978 @default.
• W2106043220 hasConceptScore W2106043220C24530287 @default.
• W2106043220 hasConceptScore W2106043220C2779832904 @default.
• W2106043220 hasConceptScore W2106043220C41625074 @default.
• W2106043220 hasConceptScore W2106043220C50952357 @default.
• W2106043220 hasConceptScore W2106043220C53345823 @default.
• W2106043220 hasConceptScore W2106043220C537181965 @default.
• W2106043220 hasConceptScore W2106043220C55493867 @default.
• W2106043220 hasConceptScore W2106043220C86803240 @default.
• W2106043220 hasIssue "2" @default.
• W2106043220 hasLocation W21060432201 @default.
• W2106043220 hasLocation W21060432202 @default.

• next